Recombinant
RabMAb

Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] (ab92701)

Overview

  • Product name
    Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051]
    See all AMPK alpha 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR3051] to AMPK alpha 1 (phospho S496)
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A phospho-specific peptide corresponding to residues surrounding Serine 487 of Human AMPK alpha 1.

  • Positive control
    • 293T cell lysate; HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Applications

Our Abpromise guarantee covers the use of ab92701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
ICC/IF
  • Application notes
    ICC/IF: 1/100 - 1/250.
    IP: 1/10 - 1/100.
    WB: 1/1000 - 1/10000. Predicted molecular weight: 64 kDa.

    Is unsuitable for Flow Cyt or IHC-P.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
    • Sequence similarities
      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
      Contains 1 protein kinase domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • 5 AMP activated protein kinase alpha 1catalytic subunit antibody
      • 5 AMP activated protein kinase catalytic alpha 1 chain antibody
      • 5' AMP activated protein kinase catalytic subunit alpha 1 antibody
      • 5'-AMP-activated protein kinase catalytic subunit alpha-1 antibody
      • AAPK1 antibody
      • AAPK1_HUMAN antibody
      • ACACA kinase antibody
      • acetyl CoA carboxylase kinase antibody
      • AI194361 antibody
      • AI450832 antibody
      • AL024255 antibody
      • AMP -activate kinase alpha 1 subunit antibody
      • AMP-activated protein kinase, catalytic, alpha -1 antibody
      • AMPK 1 antibody
      • AMPK alpha 1 antibody
      • AMPK alpha 1 chain antibody
      • AMPK antibody
      • AMPK subunit alpha-1 antibody
      • AMPK1 antibody
      • AMPKa1 antibody
      • AMPKalpha1 antibody
      • C130083N04Rik antibody
      • cb116 antibody
      • EC 2.7.11.1 antibody
      • HMG CoA reductase kinase antibody
      • HMGCR kinase antibody
      • hormone sensitive lipase kinase antibody
      • Hydroxymethylglutaryl CoA reductase kinase antibody
      • im:7154392 antibody
      • kinase AMPK alpha1 antibody
      • MGC33776 antibody
      • MGC57364 antibody
      • OTTHUMP00000161795 antibody
      • OTTHUMP00000161796 antibody
      • PRKAA 1 antibody
      • PRKAA1 antibody
      • Protein kinase AMP activated alpha 1 catalytic subunit antibody
      • SNF1-like protein AMPK antibody
      • SNF1A antibody
      • Tau protein kinase PRKAA1 antibody
      • wu:fa94c10 antibody
      see all

    Images

    • All lanes : Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] (ab92701) at 1/1000 dilution

      Lane 1 : 293T cell lysate untreated
      Lane 2 : 293T cell lysate treated with LP

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 64 kDa
    • Immunofluorescent staining of AMPK alpha 1 in HeLa cells using ab92701 at 1/100 dilution.

    References

    This product has been referenced in:
    • Wang YG & Yang TL Liraglutide reduces fatty degeneration in hepatic cells via the AMPK/SREBP1 pathway. Exp Ther Med 10:1777-1783 (2015). WB ; Human . Read more (PubMed: 26640549) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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