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Rabbit monoclonal [EPR3051] to AMPK alpha 1 (phospho S496)
ICC/IF more details Unsuitable for:
Flow Cyt or IHC-P
A phospho-specific peptide corresponding to residues surrounding Serine 487 of Human AMPK alpha 1.
293T cell lysate; HeLa cells
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/100 - 1/250.
IP: 1/10 - 1/100.
WB: 1/1000 - 1/10000. Predicted molecular weight: 64 kDa.
Is unsuitable for Flow Cyt or IHC-P.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 protein kinase domain.
Information by UniProt
5 AMP activated protein kinase alpha 1catalytic subunit antibody
5 AMP activated protein kinase catalytic alpha 1 chain antibody
5' AMP activated protein kinase catalytic subunit alpha 1 antibody
Western blot - Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] (ab92701)
All lanes : Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] (ab92701) at 1/1000 dilution Lane 1 : 293T cell lysate untreated Lane 2 : 293T cell lysate treated with LP Lysates/proteins at 10 µg per lane. Secondary All lanes : HRP-labeled goat anti-rabbit at 1/2000 dilution Predicted band size: 64 kDa
Immunocytochemistry/ Immunofluorescence - Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] (ab92701)
Immunofluorescent staining of AMPK alpha 1 in HeLa cells using ab92701 at 1/100 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"