Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody (ab23875)

Overview

  • Product nameAnti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody
  • Description
    Rabbit polyclonal to AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172)
  • Specificityab23875 recognises the phosphorylated forms of AMPK alpha 1 (T172) and AMPK alpha 2 (T183).
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human AMPK alpha 1 (phospho T183). Also within AMPK alpha 2 (phospho T172).

  • Positive control
    • Insulin treated CHO T cells, Insulin treated 3T3L1, Metformin treated L6 myoblast cells. Metformin treated HepG2 cells (10 mM for 24 hr).

Properties

Applications

Our Abpromise guarantee covers the use of ab23875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 62 kDa.

Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody images

  • All lanes : Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody (ab23875)

    Lane 1 : Lysates prepared from HepG2 cells left unstimulated with 3% BSA-TBST buffer and no peptide
    Lane 2 : Lysates prepared from HepG2 cells stimulated with Metformin with 3% BSA-TBST buffer and no peptide
    Lane 3 : Lysates prepared from HepG2 cells stimulated with Metformin with 3% BSA-TBST buffer and the non-phosphopeptide corresponding to the immunogen
    Lane 4 : Lysates prepared from HepG2 cells stimulated with Metformin with 3% BSA-TBST buffer and a generic phospho-threonine-containing peptide
    Lane 5 : Lysates prepared from HepG2 cells stimulated with Metformin with 3% BSA-TBST buffer and the phosphopeptide immunogen
    Lane 6 : Lysates prepared from HepG2 cells stimulated with Metformin and treated with lambda phosphatase with 3% BSA-TBST buffer

    Secondary
    goat F(ab’)2 anti rabbit IgG HRP conjugate

    Predicted band size : 62 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 hours

References for Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody (ab23875)

ab23875 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain Tissue)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Specification Brain Tissue
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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Submitted Oct 26 2016

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Permeabilization Yes - 0.2% Triton X100.2% Triton X100
Specification Brain
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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Verified customer

Submitted Sep 21 2016

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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I have been informed that to detect phospho AMPK, metformin was used at 10 mM for 24 hr in treating HepG2 cells. I was unable to have further information regarding the medium unfortunately, but I hope the above information will help you,

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"