Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody (ab72845)


  • Product nameAnti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody
  • Description
    Rabbit polyclonal to AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172)
  • SpecificityAntibody detects endogenous AMPK alpha only when phosphorylated at SDGEFLR(pT)SCGSPNY. The antibody detects both alpha1 and alpha2 isoforms.
  • Tested applicationsSuitable for: WB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Chicken, Human
    Predicted to work with: Mouse, Cow, DogDoes not react with: Drosophila melanogaster
  • Immunogen

    Synthetic peptide corresponding to Human AMPK alpha 1 (phospho T183). Also within AMPK alpha 2 (phospho T172). It is identical among Human, mouse, rat, chicken, bovine, dog, and insect species.

  • Positive control
    • Serum starved HEK293 whole cell lysate.


Our Abpromise guarantee covers the use of ab72845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
IP Use a concentration of 2 - 5 µg/ml.
ELISA Use a concentration of 0.01 - 0.1 µg/ml.

Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody images

  • All lanes : Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody (ab72845) at 1/500 dilution

    Lane 1 : Molecular weight markers
    Lane 2 : Whole cell lysate from serum starved HEK 293 cells
    Lane 3 : BSA negative control

    Predicted band size : 63 kDa
    Observed band size : 63 kDa

References for Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody (ab72845)

This product has been referenced in:
  • Zou D  et al. Protective effects of myricetin on acute hypoxia-induced exercise intolerance and mitochondrial impairments in rats. PLoS One 10:e0124727 (2015). WB ; Rat . Read more (PubMed: 25919288) »
  • Garvey SM  et al. Molecular and metabolomic effects of voluntary running wheel activity on skeletal muscle in late middle-aged rats. Physiol Rep 3:N/A (2015). WB ; Rat . Read more (PubMed: 25716928) »

See all 7 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Fruit fly (Drosophila melanogaster) Tissue lysate - whole (0-4hr embryos)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 30 µg
Treatment control shRNA and SNF1A shRNA embryos
Specification 0-4hr embryos
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Dr. Richelle Sopko

Verified customer

Submitted Mar 04 2014

free of charge replacement sent.

Thank you for calling Abcam earlier today.
I am sorry about the issues you have been having with ab72845 in western blot. As we discussed I am sending you a new vial of the antibody and you should receive that tomorrow morning. For your reference, ...

Read More

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

Read More

Gracias por contactarnos y tomaros la molestia de responder nuestro cuestionario.   He estudiado el protocolo enviado, y hay una serie de sugerencias que me gustaría haceros para intentar mejorar los resultados del anticuerpo. De cualquier manera, al e...

Read More