Recombinant Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5683] to AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172)
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Drosophila melanogaster
Related conjugates and formulations
Overview
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Product name
Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683]
See all AMPK alpha 1+AMPK alpha 2 primary antibodies -
Description
Rabbit monoclonal [EPR5683] to AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) -
Host species
Rabbit -
Specificity
This antibody only detects AMPK alpha 1 phosphorylated at Threonine 183 and AMPK alpha 2 phosphorylated Threonine 172.
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Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Rat, Human, Drosophila melanogaster -
Immunogen
Synthetic peptide within Human AMPK alpha 1 (phospho T183). The exact sequence is proprietary. Also within AMPK alpha 2 (phospho T172).
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Positive control
- WB: HEK-293 and MOLT-4 whole cell lysates; Mouse heart lysate; Rat brain lysate;
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 1.22 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5683 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (4) |
1/1000 - 1/10000. Detects a band of approximately 62,63 kDa (predicted molecular weight: 64 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 62,63 kDa (predicted molecular weight: 64 kDa). |
Target
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Cellular localization
AMPK alpha 2: Cytoplasm. Nucleus. In response to stress, recruited by p53/TP53 to specific promoters. -
Database links
- Entrez Gene: 5562 Human
- Entrez Gene: 5563 Human
- Entrez Gene: 105787 Mouse
- Entrez Gene: 108079 Mouse
- Entrez Gene: 65248 Rat
- Entrez Gene: 78975 Rat
- Omim: 600497 Human
- Omim: 602739 Human
see all
Images
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All lanes : Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) at 1/2000 dilution
Lane 1 : HEK-293 (human embryonic kidney) whole cell lysates
Lane 2 : HEK-293 (human embryonic kidney) whole cell lysates plus AMPK alpha 1 phospho pT183 and AMPK alpha 2 phospho pT172 peptides
Lane 3 : HEK-293 (human embryonic kidney) whole cell lysates plus AMPK alpha 1 and AMPK alpha 2 non-phospho peptides
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG, (H+L), HRP conjugated (ab97051) at 1/20000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDa
Exposure time: 15 secondsBlocking and dilution buffer: 5% NFDM/TBST
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Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) at 1/1000 dilution (purified) + Fruit fly lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 64 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
All lanes : Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) at 1/1000 dilution (purified)
Lane 1 : Mouse heart lysate
Lane 2 : Rat brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 64 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) at 1/1000 dilution (purified) + Molt-4 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 64 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
All lanes : Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) at 1/1000 dilution (purified)
Lane 1 : Untreated HEK-293 cell lysate
Lane 2 : HEK-293 cell lysate treated with Lambda phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Predicted band size: 64 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
All lanes : Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) at 1/1000 dilution (unpurified)
Lane 1 : 293 cell lysate
Lane 2 : Lysate of 293 cells treated with lambda phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 64 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (125)
ab133448 has been referenced in 125 publications.
- Hou D et al. Uncoupling protein 1 knockout aggravates isoproterenol-induced acute myocardial ischemia via AMPK/mTOR/PPARα pathways in rats. Transgenic Res 31:107-118 (2022). PubMed: 34709566
- Ji R et al. Salidroside alleviates oxidative stress and apoptosis via AMPK/Nrf2 pathway in DHT-induced human granulosa cell line KGN. Arch Biochem Biophys 715:109094 (2022). PubMed: 34813774
- Park CH et al. Canagliflozin protects against cisplatin-induced acute kidney injury by AMPK-mediated autophagy in renal proximal tubular cells. Cell Death Discov 8:12 (2022). PubMed: 35013111
- Chen M et al. Metformin protects lens epithelial cells against senescence in a naturally aged mouse model. Cell Death Discov 8:8 (2022). PubMed: 35013152
- Yang J et al. Pitavastatin activates mitophagy to protect EPC proliferation through a calcium-dependent CAMK1-PINK1 pathway in atherosclerotic mice. Commun Biol 5:124 (2022). PubMed: 35145192