Anti-AMPK beta 1 antibody (ab133213)

Overview

  • Product name
  • Description
    Rabbit polyclonal to AMPK beta 1
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide from internal sequence of Human AMPK beta 1.

  • Positive control
    • Human colon tissue. HepG2, Jurkat, HuvEc and MCF-7 extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab133213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 30 kDa.
ELISA 1/5000.
IHC-P Use a concentration of 10 µg/ml.

Target

  • Function
    Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
  • Sequence similarities
    Belongs to the 5'-AMP-activated protein kinase beta subunit family.
  • Domain
    The glycogen-binding domain may target AMPK to glycogen so that other factors like glycogen-bound debranching enzyme or protein phosphatases can directly affect AMPK activity.
  • Post-translational
    modifications
    Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1300015D22Rik antibody
    • 5''-AMP-activated protein kinase subunit beta-1 antibody
    • 5'-AMP-activated protein kinase beta-1 subunit antibody
    • AAKB1_HUMAN antibody
    • AMP-activated protein kinase beta subunit antibody
    • AMP-ACTIVATED PROTEIN KINASE, NONCATALYTIC, BETA-1 antibody
    • AMP-activated, noncatalytic, beta-1 antibody
    • AMPK antibody
    • AMPK beta 1 chain antibody
    • AMPK subunit beta-1 antibody
    • AMPK-BETA-1 antibody
    • AMPKb antibody
    • AU021155 antibody
    • E430008F22 antibody
    • HAMPKb antibody
    • MGC17785 antibody
    • PRKAB1 antibody
    • Protein kinase AMP activated non catalytic subunit beta 1 antibody
    • protein kinase, AMP-activated, beta 1 non-catalytic subunit antibody
    • protein kinase, AMP-activated, noncatalytic, beta-1 antibody
    see all

Anti-AMPK beta 1 antibody images

  • All lanes : Anti-AMPK beta 1 antibody (ab133213) at 1/500 dilution

    Lane 1 : Extract from HepG2 cells
    Lane 2 : Extract from Jurkat cells
    Lane 3 : Extract from HuvEc cells
    Lane 4 : Extract from MCF-7 cells
    Lane 5 : Extract from HuvEc cells with immunizing peptide


    Predicted band size : 30 kDa
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon labelling AMPK beta 1 with ab133213 at 10 µg/ml, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References for Anti-AMPK beta 1 antibody (ab133213)

ab133213 has not yet been referenced specifically in any publications.

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I ran the alignment. There is one 13 amino acid stretch that has 85% identity, however, the overall homology, is only 65% between the human immunogen and the corresponding region in Zebrafish. So unfortunately I do...

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