Anti-AMPK beta 1 (phospho S108) antibody (ab156890)


  • Product name
    Anti-AMPK beta 1 (phospho S108) antibody
    See all AMPK beta 1 primary antibodies
  • Description
    Rabbit polyclonal to AMPK beta 1 (phospho S108)
  • Host species
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Sheep, Horse, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human AMPK beta 1 aa 100-200 (phospho S108) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q9Y478

  • Positive control
    • This antibody gave a positive signal in HEK293 Oligomycin 1µM whole cell lysate.



Our Abpromise guarantee covers the use of ab156890 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Detects a band of approximately 38 kDa (predicted molecular weight: 30 kDa).
ELISA Use a concentration of 1 µg/ml.


  • Function
    Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
  • Sequence similarities
    Belongs to the 5'-AMP-activated protein kinase beta subunit family.
  • Domain
    The glycogen-binding domain may target AMPK to glycogen so that other factors like glycogen-bound debranching enzyme or protein phosphatases can directly affect AMPK activity.
  • Post-translational
    Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1300015D22Rik antibody
    • 5''-AMP-activated protein kinase subunit beta-1 antibody
    • 5'-AMP-activated protein kinase beta-1 subunit antibody
    • AAKB1_HUMAN antibody
    • AMP-activated protein kinase beta subunit antibody
    • AMP-activated, noncatalytic, beta-1 antibody
    • AMPK antibody
    • AMPK beta 1 chain antibody
    • AMPK subunit beta-1 antibody
    • AMPK-BETA-1 antibody
    • AMPKb antibody
    • AU021155 antibody
    • E430008F22 antibody
    • HAMPKb antibody
    • MGC17785 antibody
    • PRKAB1 antibody
    • Protein kinase AMP activated non catalytic subunit beta 1 antibody
    • protein kinase, AMP-activated, beta 1 non-catalytic subunit antibody
    • protein kinase, AMP-activated, noncatalytic, beta-1 antibody
    see all


  • All lanes : Anti-AMPK beta 1 (phospho S108) antibody (ab156890) at 1/250 dilution

    Lane 1 : Human HEK293 Oligomycin 1µM
    Lane 2 : Human HEK293 Oligomycin 1µM with Immunising peptide at 1/250 dilution
    Lane 3 : Human HEK293 Oligomycin 1µM with Control peptide at 1/250 dilution

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 30 kDa
    Observed band size: 38 kDa (why is the actual band size different from the predicted?)

    Exposure time: 2 minutes

    The predicted molecular weight of AMPK beta 1 is 30 kDa (SwissProt), however we expect to observe a banding pattern at 38 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab156890 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406

  • ab156890 was tested using an Indirect ELISA approach. The wells were coated with peptide (1µg/ml at 100µl/well) overnight at 4°C, followed by a 5% BSA blocking step for 1 hour at room temperature. The primary Ab was then added at a dilution range of 1- 0.00025µg/ml (100µl/well) for 1hr at room temperature. A HRP-conjugated anti-rabbit IgG (heavy and light chain) was used as a secondary antibody at 1:20,000 dilution for 1hr at room temperature.


ab156890 has not yet been referenced specifically in any publications.

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