Anti-AMPK beta 1 (phospho S181) antibody (ab55311)

Overview

  • Product name
    Anti-AMPK beta 1 (phospho S181) antibody
    See all AMPK beta 1 primary antibodies
  • Description
    Rabbit polyclonal to AMPK beta 1 (phospho S181)
  • Specificity
    This antibody detects endogenous levels of AMPK beta 1 only when phosphorylated at serine 181.
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from human AMPK beta 1 around the phosphorylation site of serine 181 (S-S-SP-P-P).

  • Positive control
    • Human skeletal muscle tissue, extracts from Jurkat cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab55311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
WB 1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 30 kDa).
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
  • Sequence similarities
    Belongs to the 5'-AMP-activated protein kinase beta subunit family.
  • Domain
    The glycogen-binding domain may target AMPK to glycogen so that other factors like glycogen-bound debranching enzyme or protein phosphatases can directly affect AMPK activity.
  • Post-translational
    modifications
    Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1300015D22Rik antibody
    • 5''-AMP-activated protein kinase subunit beta-1 antibody
    • 5'-AMP-activated protein kinase beta-1 subunit antibody
    • AAKB1_HUMAN antibody
    • AMP-activated protein kinase beta subunit antibody
    • AMP-ACTIVATED PROTEIN KINASE, NONCATALYTIC, BETA-1 antibody
    • AMP-activated, noncatalytic, beta-1 antibody
    • AMPK antibody
    • AMPK beta 1 chain antibody
    • AMPK subunit beta-1 antibody
    • AMPK-BETA-1 antibody
    • AMPKb antibody
    • AU021155 antibody
    • E430008F22 antibody
    • HAMPKb antibody
    • MGC17785 antibody
    • PRKAB1 antibody
    • Protein kinase AMP activated non catalytic subunit beta 1 antibody
    • protein kinase, AMP-activated, beta 1 non-catalytic subunit antibody
    • protein kinase, AMP-activated, noncatalytic, beta-1 antibody
    see all

Images

  • This image shows human skeletal muscle tissue stained with ab55311 at 1/50 dilution. The left hand image shows untreated tissue, the right hand image shows tissue treated with the immunising phosphopeptide.
  • All lanes : Anti-AMPK beta 1 (phospho S181) antibody (ab55311) at 1/500 dilution

    Lane 1 : Jurkat cell extract
    Lane 2 : Jurkat cell extract, with the immunising phosphopeptide.


    Predicted band size : 30 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)

References

ab55311 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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