Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (ab68206)


  • Product nameAnti-AMPK beta 1 (phospho S181) antibody [EP2181Y]
    See all AMPK beta 1 primary antibodies
  • Description
    Rabbit monoclonal [EP2181Y] to AMPK beta 1 (phospho S181)
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic phospho-peptide corresponding to residues surrounding Serine 181 of human AMPK beta 1.

  • Positive control
    • Human brain tissue lysate (cells untreated or treated with AP), HeLa cells and human skeletal muscle tissue
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


Associated products


Our Abpromise guarantee covers the use of ab68206 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/2000 - 1/20000. Detects a band of approximately 32, 45 kDa (predicted molecular weight: 30 kDa).
IHC-P 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionNon-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
    • Sequence similaritiesBelongs to the 5'-AMP-activated protein kinase beta subunit family.
    • DomainThe glycogen-binding domain may target AMPK to glycogen so that other factors like glycogen-bound debranching enzyme or protein phosphatases can directly affect AMPK activity.
    • Post-translational
      Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK.
    • Information by UniProt
    • Database links
    • Alternative names
      • 1300015D22Rik antibody
      • 5''-AMP-activated protein kinase subunit beta-1 antibody
      • AAKB1_HUMAN antibody
      • AMP-activated, noncatalytic, beta-1 antibody
      • AMPK antibody
      • AMPK beta 1 chain antibody
      • AMPK subunit beta-1 antibody
      • AMPK-BETA-1 antibody
      • AMPKb antibody
      • AU021155 antibody
      • E430008F22 antibody
      • HAMPKb antibody
      • MGC17785 antibody
      • PRKAB1 antibody
      • Protein kinase AMP activated non catalytic subunit beta 1 antibody
      see all

    Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] images

    • All lanes : Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (ab68206) at 1/20000 dilution

      Lane 1 : human brain tissue lysate (cells were untreated)
      Lane 2 : human brain tissue lysate (cells were treated with AP)

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 30 kDa
      Observed band size : 32 and 45 kDa (why is the actual band size different from the predicted?)
    • Immunofluorescent staining of HeLa cells using ab68206 at 1/100-1/250 dilution.
    • Immunohistochemical analysis of paraffin-embedded human skeletal muscle using ab68206 at 1/100-1/250 dilution.

    References for Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (ab68206)

    ab68206 has not yet been referenced specifically in any publications.

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