• Product nameAnti-AMSH antibody
    See all AMSH primary antibodies
  • Description
    Rabbit polyclonal to AMSH
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Non Human Primates
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human AMSH.

    (Peptide available as ab74470.)

  • Positive control
    • This antibody gave a positive signal in the following lysates: HeLa Whole Cell HepG2 Whole Cell SW480 Whole Cell Rat Colon Tissue



Our Abpromise guarantee covers the use of ab65281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa).


  • FunctionZinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not cleave 'Lys-48'-linked polyubiquitin chains (By similarity). Functions at the endosome and is able to oppose the ubiquitin-dependent sorting of receptors to lysosomes. Plays a role in signal transduction for cell growth and MYC induction mediated by IL-2 and GM-CSF. Potentiates BMP (bone morphogenetic protein) signaling by antagonizing the inhibitory action of SMAD6 and SMAD7.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the peptidase M67C family.
    Contains 1 MPN (JAB/Mov34) domain.
  • DomainThe JAMM motif is essential for the protease activity.
  • Post-translational
    Phosphorylated after BMP type I receptor activation.
    Ubiquitinated by SMURF2 in the presence of RNF11.
  • Cellular localizationNucleus. Membrane. Cytoplasm. Early endosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • AMSH antibody
    • Associated molecule with the SH3 domain of STAM antibody
    • Endosome associated ubiquitin isopeptidase antibody
    • Endosome-associated ubiquitin isopeptidase antibody
    • MGC126516 antibody
    • MGC126518 antibody
    • MICCAP antibody
    • STABP_HUMAN antibody
    • STAM binding protein antibody
    • STAM-binding protein antibody
    • Stambp antibody
    see all

Anti-AMSH antibody images

  • All lanes : Anti-AMSH antibody (ab65281) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : Colon (Rat) Tissue Lysate
    Lane 4 : SW480 whole cell lysate (ab3957)

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 102 kDa (possible post-translational modification),55 kDa (possible post-translational modification).

    Exposure time : 30 seconds
  • ICC/IF image of ab65821 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65281, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
  • IHC image of AMSH staining in Human Cerebral Cortex FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab65281, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

References for Anti-AMSH antibody (ab65281)

This product has been referenced in:

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab65281.
Please use the links above to contact us or submit feedback about this product.