Overview

  • Product nameAnti-AMSH antibody [EPR4361]
    See all AMSH primary antibodies
  • Description
    Rabbit monoclonal [EPR4361] to AMSH
  • Tested applicationsSuitable for: WB, ICCmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide, corresponding to residues in human AMSH

  • Positive control
    • Jurkat cell lysate, HeLa cell lysate, Raji cell lysate, SW480 cell lysate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab108301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
ICC 1/100 - 1/250.

Target

  • FunctionZinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not cleave 'Lys-48'-linked polyubiquitin chains (By similarity). Functions at the endosome and is able to oppose the ubiquitin-dependent sorting of receptors to lysosomes. Plays a role in signal transduction for cell growth and MYC induction mediated by IL-2 and GM-CSF. Potentiates BMP (bone morphogenetic protein) signaling by antagonizing the inhibitory action of SMAD6 and SMAD7.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the peptidase M67C family.
    Contains 1 MPN (JAB/Mov34) domain.
  • DomainThe JAMM motif is essential for the protease activity.
  • Post-translational
    modifications
    Phosphorylated after BMP type I receptor activation.
    Ubiquitinated by SMURF2 in the presence of RNF11.
  • Cellular localizationNucleus. Membrane. Cytoplasm. Early endosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • AMSH antibody
    • Associated molecule with the SH3 domain of STAM antibody
    • Endosome associated ubiquitin isopeptidase antibody
    • Endosome-associated ubiquitin isopeptidase antibody
    • MGC126516 antibody
    • MGC126518 antibody
    • MICCAP antibody
    • STABP_HUMAN antibody
    • STAM binding protein antibody
    • STAM-binding protein antibody
    • Stambp antibody
    see all

Anti-AMSH antibody [EPR4361] images

  • All lanes : Anti-AMSH antibody [EPR4361] (ab108301) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Raji cell lysate
    Lane 4 : SW480 cell lysate

    Lysates/proteins at 10 µg per lane.


    Performed under reducing conditions.

    Predicted band size : 48 kDa

References for Anti-AMSH antibody [EPR4361] (ab108301)

ab108301 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"