Amthamine dihydrobromide (ab120778)

Overview

  • Product nameAmthamine dihydrobromide
  • Description
    Selective H2 agonist
  • Biological descriptionSelective H2 agonist (pKi = 5.2). Weak antagonist of H3 and displays no activity at H1. Increases gastric acid secretion. Additionally increases blood pressure.
  • Purity> 99%

Properties

    Applications

    Our Abpromise guarantee covers the use of ab120778 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Amthamine dihydrobromide images

    • ab78079 staining PKA R2 (phospho S96) in HepG2 cells treated with amthamine dihydrobromide (ab120778), by ICC/IF. Increase of PKA R2 (phospho S96) expression correlates with increased concentration of amthamine dihydrobromide, as described in literature.
      The cells were incubated at 37°C for 60 minutes in media containing different concentrations of ab120778 (amthamine dihydrobromide) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab78079 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    References for Amthamine dihydrobromide (ab120778)

    This product has been referenced in:
    • Lim HD  et al. Evaluation of histamine H1-, H2-, and H3-receptor ligands at the human histamine H4 receptor: identification of 4-methylhistamine as the first potent and selective H4 receptor agonist. J Pharmacol Exp Ther 314:1310-21 (2005). Read more (PubMed: 15947036) »
    • Poli E  et al. In vitro cardiac pharmacology of the new histamine H2-receptor agonist amthamine: comparisons with histamine and dimaprit. Agents Actions 40:44-9 (1993). Read more (PubMed: 8147269) »
    • Coruzzi G  et al. The new potent and selective histamine H2 receptor agonist amthamine as a tool to study gastric secretion. Naunyn Schmiedebergs Arch Pharmacol 348:77-81 (1993). Read more (PubMed: 8377843) »

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    Product Wall

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be use...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"