Overview

  • Product name
  • Description
    Rabbit polyclonal to Amylin
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Cat, Dog, Baboon
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Amylin.

  • Positive control
    • This antibody gave a positive signal in a human pancreas tissue section.

Properties

Applications

Our Abpromise guarantee covers the use of ab77387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    Selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism.
  • Sequence similarities
    Belongs to the calcitonin family.
  • Domain
    The mature protein is largely unstructured in the absence of a cognate ligand, and has a strong tendency to form fibrillar aggregates. Homodimerization may be the first step of amyloid formation.
  • Post-translational
    modifications
    Amyloid fibrils are degraded by IDE.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Amylin antibody
    • DAP antibody
    • Diabetes associated peptide antibody
    • Diabetes-associated peptide antibody
    • IAP antibody
    • IAPP antibody
    • IAPP_HUMAN antibody
    • Insulinoma amyloid peptide antibody
    • Islet amyloid polypeptide (diabetes associated peptide; amylin) antibody
    • Islet amyloid polypeptide antibody
    see all

Images

  • IHC image of Amylin staining in Human Pancreas section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77387, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

References

ab77387 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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