Overview

  • Product name
    Anti-Amylin antibody [R10/99]
    See all Amylin primary antibodies
  • Description
    Mouse monoclonal [R10/99] to Amylin
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Guinea pig, Cat, Dog, Chinese hamster
  • Immunogen

    Synthetic peptide:

    CATQRLANFLV

    , corresponding to amino acids 40-50 of Human Amylin coupled to tuberculin.

  • Positive control
    • Human Pancreas, Islet of Langerhans

Properties

Applications

Our Abpromise guarantee covers the use of ab115766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • ab115766, at 1/100 staining Amylin in formalin fixed, paraffin embedded Human Pancreas, Islet of Langerhans by Immunohistochemistry. Developed using biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase streptavidin and chromogen.

References

This product has been referenced in:
  • Kong S  et al. Kill two birds with one stone: making multi-transgenic pre-diabetes mouse models through insulin resistance and pancreatic apoptosis pathogenesis. PeerJ 6:e4542 (2018). Read more (PubMed: 29682407) »
  • Rössger K  et al. A closed-loop synthetic gene circuit for the treatment of diet-induced obesity in mice. Nat Commun 4:2825 (2013). Read more (PubMed: 24281397) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
ELISA
Sample
Rat Recombinant protein (Synthetic rat amylin peptide)
Specification
Synthetic rat amylin peptide
Blocking step
Pierce Protein Free T20 (PBS) Blocking Buffer #37573 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C
Type
Sandwich (Detection)
Username

Abcam user community

Verified customer

Submitted Mar 07 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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