The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionSelectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism.
Sequence similaritiesBelongs to the calcitonin family.
DomainThe mature protein is largely unstructured in the absence of a cognate ligand, and has a strong tendency to form fibrillar aggregates. Homodimerization may be the first step of amyloid formation.
Post-translational modificationsAmyloid fibrils are degraded by IDE.
ab115766, at 1/100 staining Amylin in formalin fixed,
paraffin embedded Human Pancreas, Islet of
Langerhans by Immunohistochemistry. Developed using biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase streptavidin and chromogen.
References for Anti-Amylin antibody [R10/99] (ab115766)
This product has been referenced in:
Rössger K et al. A closed-loop synthetic gene circuit for the treatment of diet-induced obesity in mice. Nat Commun4:2825 (2013).
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