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Western blot - Amyloid beta precursor protein antibody (ab12269)
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Product Name
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Amyloid beta precursor protein antibody
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See all Amyloid beta precursor protein antibodies (13)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Amyloid beta precursor protein
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Immunogen
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Synthetic peptide from within human KPI domain of human APP751/770 conjugated to Keyhole Limpet Heamocyanin (KLH)
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Reacts with
(species key)
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Hu, Rat, Mcq
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Specificity
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APP-KPI antibody (ab12269) recognises both APP751 and APP770 as both APP751 and APP770 contain the KPI domain.
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Tested applications
(see key)
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ICC, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC: 1/100 - 1/500; requires denatured and reduced sample to allow access to epitope.
IHC-P: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution; requires denatured and reduced sample to allow access to epitope.
WB: 1/500 - 1/2000. Detects a band of approximately 116 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Type I membrane protein.
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Research areas
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Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Amyloid
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Relevance
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Amyloid beta precursor protein gene (ABPP) encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Multiple transcript variants encoding several different isoforms have been found for this gene. Isoform APP695 is the predominant form in neuronal tissue, isoform APP751 and isoform APP770 are widely expressed in nonneuronal cells. Isoform APP751 is the most abundant form in T lymphocytes. ABPP is expressed in all fetal tissues examined with the highest levels in brain, kidney, heart and spleen with weak expression observed in liver; ABPP is induced during neuronal differentiation. In the adult brain, highest expression of ABPP gene is found in the frontal lobe of the cortex and in the anterior perisylvian cortex opercular gyri; moderate expression in the cerebellar cortex, the posterior perisylvian cortex opercular gyri and the temporal associated cortex. Weak expression is found in the striate, extra striate and motor cortices. Mutations in ABPP have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: Whole serum Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Amyloid beta precursor protein antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Amyloid beta precursor protein antibody images, references, products related to ab12269 and other tools.
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Amyloid beta precursor protein antibody images:
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 Western blot - Amyloid beta precursor protein antibody (ab12269)
Soluble extract of macaque brain (Macaca fasicularis, lanes 1, 3, 5) or conditioned medium from CHO cells expressing recombinant human APP770 (lanes 2, 4, 6) were separated by 7.5% SDS-PAGE, transferred to nitrocellulose and immunoblotted with the following antibodies: Lanes 1 and 2 = ab12266 mouse monoclonal [DE2B4] antibody to b-amyloid 1-17 of APP
Lanes 3 and 4 = ab12269 rabbit APP-KPI antibody
Lanes 5 and 6 = ab12268 rabbit anti APP-OX2 antibody Interpretation of Legend: DE2B4 recognises an epitope present in soluble APP cleaved by α-secretase (hence APPsα). (N.B. this epitope is also present at the N-terminus of β-amyloid peptide). In consequence, DE2B4 recognises soluble forms of both APP695 and APP751/770 after cleavage by α-secretase. This gel system does not resolve APP751 from APP770. The anti-APP-KPI antibody recognises the mixture of APP751/770 since both APP751 and APP770 contain the KPI domain but does not distinguish between them. The OX2 domain is only present in APP770 so the anti-APP-OX2 antibody recognises the recombinant APP770 while the lack of staining of the monkey brain extract suggests the level of APP770 is very low and the band stained by anti-APP-KPI in the monkey brain extract is probably APP751
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References for Amyloid beta precursor protein antibody (ab12269)
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This product has been used in: (two most recent references)
Hiltunen M et al. Focal cerebral ischemia in rats alters APP processing and expression of Abeta peptide degrading enzymes in the thalamus. Neurobiol Dis 35:103-13 (2009). WB; Rat. PubMed: 19426802
Campbell E et al. Methods to uncover an antibody epitope in the KPI domain of Alzheimer's amyloid precursor protein for immunohistochemistry in human brain. J Neurosci Methods 93:133-8 (1999). PubMed: 10634498
If you publish research using ab12269 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Amyloid beta precursor protein
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