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AbcamBiochemicals (Asc-087)

Anandamide (ethanol solution) (ab120087)

Submit a Question Specific References (1)

Overview

  • Product nameAnandamide (ethanol solution)
  • Description
    Endogenous cannabinoid
  • Alternative names
    AEA Arachidonylethanolamide
  • Biological descriptionEndogenous cannabinoid, binding to CB1, CB2 and vanilloid receptors. Cannabimimetic in vivo.
  • Purity> 98%

    • Properties

  • Chemical name(5Z,8Z,11Z,14Z)-N-(2-Hydroxyethyl)icosa-5,8,11,14-tetraenamide
  • Molecular Weight347.54
  • Chemical structureChemical Structure
  • Molecular formulaC22H37NO2
  • CAS Number94421-68-8
  • PubChem identifier5281969
  • Storage instructionsStore at -20°C (desiccating conditions).
  • Solubility overviewSupplied in ethanol (5 mg/ml)
  • Handling

    Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 6 months. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

  • SMILESO=C(CCC/C=CC/C=CC/C=CC/C=CCCCCC)NCCO
  • Source

    Synthetic

  • Research Areas

    • Anandamide (ethanol solution) images

    • Functional Studies-Anandamide (ethanol solution)(ab120087)
      Functional Studies-Anandamide (ethanol solution)(ab120087)
      ab81298 staining FAK (phospho Y397) in SK-N-SH cells treated with anandamide (ethanol solution) (ab120087), by ICC/IF. Increase in FAK (phospho Y397) expression correlates with increased concentration of anandamide (ethanol solution), as described in literature.
      The cells were incubated at 37°C for 10 minutes in media containing different concentrations of ab120087 (anandamide (ethanol solution)) in ethanol, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab81298 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue. Membranes are stained in red with WGA.

    References for Anandamide (ethanol solution) (ab120087)

    This product has been referenced in:
    • Wang X  et al. Significant neuroprotection against ischemic brain injury by inhibition of the MEK1 protein kinase in mice: exploration of potential mechanism associated with apoptosis. J Pharmacol Exp Ther 304:172-8 (2003). Read more (PubMed: 12490588) »
    • Di Marzo V  et al. Anandamide: some like it hot. Trends Pharmacol Sci 22:346-9 (2001). Read more (PubMed: 11431028) »
    • Pertwee RG Pharmacology of cannabinoid receptor ligands. Curr Med Chem 6:635-64 (1999). Read more (PubMed: 10469884) »

    See 1 Publication for this product

    Publishing research using ab120087 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"