Anti-Ancient ubiquitous protein 1 antibody (ab101984)

Overview

  • Product name
    Anti-Ancient ubiquitous protein 1 antibody
    See all Ancient ubiquitous protein 1 primary antibodies
  • Description
    Rabbit polyclonal to Ancient ubiquitous protein 1
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptice corresponding to a region between residue 426 and 476 of Human Ancient ubiquitous protein 1 (NP_853553.1).

  • Positive control
    • Hela and 293T whole cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab101984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 53 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    May play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome.
  • Tissue specificity
    Detected in blood platelets and leukocytes (at protein level). Ubiquitous. Highly expressed in placenta, liver, kidney, skeletal muscle, heart and brain.
  • Sequence similarities
    Belongs to the AUP1 family.
    Contains 1 CUE domain.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AA589454 antibody
    • Ancient ubiquitous protein 1 antibody
    • AUP 1 antibody
    • aup1 antibody
    • AUP1_HUMAN antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling Ancient ubiquitous protein 1 with ab101984 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-Ancient ubiquitous protein 1 antibody (ab101984) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 53 kDa


    Exposure time : 3 minutes
  • Detection of Ancient ubiquitous protein 1 by Western Blot of Immunprecipitate.
    Lane 1: ab101984, at 1 µg/ml, staining Ancient ubiquitous protein 1 in HeLa whole cell lysate immunoprecipitated using ab101984 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Lane 2: Control IgG
    Detection: Chemiluminescence with an exposure time of 10 seconds.

References

ab101984 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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