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Recombinant fragment corresponding to Human Androgen Receptor aa 331-572.
Our Abpromise guarantee covers the use of ab2742 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use a concentration of 5 µg/ml.|
|IHC-Fr||Use a concentration of 4 µg/ml.|
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 99 kDa).|
|Gel supershift assays||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 4 µg/ml. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.|
Immunohistochemistry was performed on normal biopsies of deparaffinized Human thyroid tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a Anti-Androgen Receptor antibody [AN1-15] (ab2742) at a dilution of 1:20 or without primary antibody (negative control) overnight at 4°C. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
ICC/IF image of ab2742 staining human prostate cells expressing androgen receptors. The cells were fixed with 4% paraformaldehyde, permeabilised with 0.8% Triton X-100, and blocked with 20% serum for 1 hour at 24°C. The primary anitibody was diluted 1/50 in 20% horse serum in PBS and incubated for 20 minutes at 4°C. An Alexa Fluor® 488 conjugated donkey anti-rat was used as the secondary antibody.
The blue staining is the cell nuclei, the green staining shows the androgen receptors.
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