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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Androgen Receptor antibody [AR 441] (ab9474)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Androgen Receptor antibody [AR 441] (ab9474)](/ps/datasheet/images/ab9474_1.jpg)
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Immunocytochemistry/ Immunofluorescence - Androgen Receptor antibody [AR 441] (ab9474)
![Immunocytochemistry/ Immunofluorescence - Androgen Receptor antibody [AR 441] (ab9474)](/ps/datasheet/images/9/ab9474/Androgen-Receptor-Primary-antibodies-ab9474-7.jpg)
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Product Name
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Androgen Receptor antibody [AR 441]
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See all Androgen Receptor antibodies (22)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [AR 441] to Androgen Receptor
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Immunogen
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Synthetic peptide: STEDTAEYSPFKGGYTK, corresponding to amino acids 299-315 of Human Androgen Receptor.
BLAST 'STEDTAEYSPFKGGYTK' with  or 
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Reacts with
(species key)
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Hu
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Specificity
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This antibody is specific to a protein of 110 kD, identified as androgen receptor.
This antibody reacts with full length androgen receptor and also with the newly described A form of the receptor.
This antibody does not cross react with estrogen, progesterone or glucocorticoid receptors.
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Tested applications
(see key)
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ICC/IF, IHC-Fr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: 1/25 - 1/50 for 30 - 60 mins at RT(ABC method). Perform heat mediated antigen retrieval (10 mM citrate buffer, pH 6.0) before commencing with IHC staining protocol.
IHC-Fr: 1/25 - 1/50 for 30 - 60 mins at RT(ABC method). Fix with acetone.
WB: 1/50 - 1/100. Detects a band of approximately 110 kDa (predicted molecular weight: 99 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Prostate Carcinoma.
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Cellular localization
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Nuclear
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Research areas
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Developmental Biology >> Reproduction >> Hormones
Cancer >> Signal transduction >> Nuclear signaling >> Nuclear hormone receptors >> Other
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Testosterone
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Testosterone
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Relevance
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Androgens have a broad range of effects on the appearance and maintenance of male secondary sexual characteristics. Like other members of the steroid superfamily, the androgen receptor (AR) consists of an amino terminal modulating domain, a central DNA binding domain, a hinge region and a carboxy terminal ligand binding domain.
The androgen receptor (AR) is a 110 kDa androgen-dependent transcription factor that is a member of the steroid/nuclear receptor gene superfamily. The AR signaling pathway plays a key role in development and function of male reproductive organs, including the prostate and epididymis. AR also plays a role in nonreproductive organs, such as muscle, hair follicles, and brain. Abnormalities in the AR signaling pathway have been linked to a number of diseases, including prostate cancer, Kennedy's disease and male infertility. The PI3K/Akt signaling pathway plays an important role in regulating AR activity through phosphorylation of AR at Ser213/210 and Ser791/790. Growth factors or cytokines may induce phosphorylation of AR through the PI3K/Akt pathway. Activation of the PI3K/AKt pathway is thought to have a survival role in prostate cancer by protecting cells from apoptosis.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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AR 441
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Isotype
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IgG1
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Purity
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Tissue culture supernatant
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Storage buffer
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n/a
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Androgen Receptor antibody [AR 441] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Androgen Receptor antibody [AR 441] images, references, products related to ab9474 and other tools.
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Androgen Receptor antibody [AR 441] images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Androgen Receptor antibody [AR 441] (ab9474)
ab9474 staining human prostate by IHC-P.
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Immunocytochemistry/ Immunofluorescence - Androgen Receptor antibody [AR 441] (ab9474)
ab9474 staining the Androgen Receptor in the human 22Rv1 Prostate Cancer Cell Line by ICC/IF (immunocytochemistry/immunofluorescence). Cells were formaldehyde fixed, permeabilized by 0.1% Triton for 15 minutes, and blocked with glycine (7.5mg/ml) for 15 minutes at room temperature. The sample was incubated with the primary antibody (1/50 in PBS) for 12 hours at 4°C. An undiluted Alexa Fluor 488®-conjugated Donkey anti-mouse polyclonal was used as the secondary. This image is courtesy of an Abreview submitted by Dr Victor Campa
See Abreview
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References for Androgen Receptor antibody [AR 441] (ab9474)
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This product has been used in: (two most recent references)
Foresta C et al. Androgens stimulate endothelial progenitor cells through an androgen receptor-mediated pathway. Clin Endocrinol (Oxf) 68:284-9 (2008). PubMed: 17803706
Bidaux G et al. Prostate cell differentiation status determines transient receptor potential melastatin member 8 channel subcellular localization and function. J Clin Invest 117:1647-57 (2007). PubMed: 17510704
See all 3 publication references for this product.
If you publish research using ab9474 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Androgen Receptor
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Androgen Receptor antibody [AR 441] - more information
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Customer reviews (feedback) regarding Androgen Receptor antibody [AR 441] |
Customer FAQs regarding Androgen Receptor antibody [AR 441] |
Protocols for Androgen Receptor antibody [AR 441] |
Price and availability of products related to Androgen Receptor antibody [AR 441] |
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Androgen Receptor antibody [AR 441] - related products:
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Androgen Receptor antibody [AR 441] - other tools:
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Contact Abcam with a Technical Enquiry about Androgen Receptor antibody [AR 441] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Androgen Receptor antibody [AR 441] (ab9474)](/index.html?datasheet=9474#ab9474_1.jpg)
![Immunocytochemistry/ Immunofluorescence - Androgen Receptor antibody [AR 441] (ab9474)](/index.html?datasheet=9474#Androgen-Receptor-Primary-antibodies-ab9474-7.jpg)
