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Fusion protein corresponding to Human Androgen Receptor aa 331-572.
Our Abpromise guarantee covers the use of ab3509 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|IHC-Fr||1/10 - 1/100.|
|IP||Use at an assay dependent concentration.|
|WB||1/500. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).|
|ICC/IF||Use at an assay dependent concentration. PubMed: 22850685|
|ChIP||Use at an assay dependent concentration.|
|IHC-P||1/10 - 1/100.|
Immunohistochemistry (Formaline/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue labeling Androgen Receptor with ab3509 at 1/20 (right). Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 minutes. Tissues were blocked in 3% H2O2-methanol for 15 minutes at room temperature. A HRP-conjugated secondary antibody was used. Left - negative control.