Anti-Androgen Receptor antibody - ChIP Grade (ab3509)

Overview

  • Product nameAnti-Androgen Receptor antibody - ChIP Grade
    See all Androgen Receptor primary antibodies
  • Description
    Rabbit polyclonal to Androgen Receptor - ChIP Grade
  • SpecificityThis antibody detects Androgen Receptor. It does not detect estrogen, progesterone, or glucocorticoid receptors. Immunohistochemical staining of AR in rat brain results in intense nuclear staining.
  • Tested applicationsSuitable for: ICC, IHC-Fr, IP, WB, ICC/IF, ChIP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Hamster, Human, Pig, Non Human Primates
  • Immunogen

    Fusion protein corresponding to Human Androgen Receptor aa 331-572.

  • Positive control
    • rat brain

Properties

Applications

Our Abpromise guarantee covers the use of ab3509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
IHC-Fr 1/10 - 1/100.
IP Use at an assay dependent concentration.
WB 1/500. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 22850685
ChIP Use at an assay dependent concentration.
IHC-P 1/10 - 1/100.

Target

  • FunctionSteroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.
    Isoform 3 and isoform 4 lack the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.
  • Tissue specificityIsoform 2 is mainly expressed in heart and skeletal muscle (PubMed:15634333). Isoform 3 is expressed by basal and stromal cells of prostate (at protein level) (PubMed:19244107).
  • Involvement in diseaseAndrogen insensitivity syndrome
    Spinal and bulbar muscular atrophy X-linked 1
    Defects in AR may play a role in metastatic prostate cancer. The mutated receptor stimulates prostate growth and metastases development despite of androgen ablation. This treatment can reduce primary and metastatic lesions probably by inducing apoptosis of tumor cells when they express the wild-type receptor.
    Androgen insensitivity, partial
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. In the presence of bound steroid the ligand-binding domain interacts with the N-terminal modulating domain, and thereby activates AR transcription factor activity. Agonist binding is required for dimerization and binding to target DNA. The transcription factor activity of the complex formed by ligand-activated AR and DNA is modulated by interactions with coactivator and corepressor proteins. Interaction with RANBP9 is mediated by both the N-terminal domain and the DNA-binding domain. Interaction with EFCAB6/DJBP is mediated by the DNA-binding domain.
  • Post-translational
    modifications
    Sumoylated on Lys-388 (major) and Lys-521. Ubiquitinated. Deubiquitinated by USP26. 'Lys-6' and 'Lys-27'-linked polyubiquitination by RNF6 modulates AR transcriptional activity and specificity.
    Phosphorylated in prostate cancer cells in response to several growth factors including EGF. Phosphorylation is induced by c-Src kinase (CSK). Tyr-535 is one of the major phosphorylation sites and an increase in phosphorylation and Src kinase activity is associated with prostate cancer progression. Phosphorylation by TNK2 enhances the DNA-binding and transcriptional activity and may be responsible for androgen-independent progression of prostate cancer. Phosphorylation at Ser-83 by CDK9 regulates AR promoter selectivity and cell growth. Phosphorylation by PAK6 leads to AR-mediated transcription inhibition.
    Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
  • Cellular localizationNucleus. Cytoplasm. Predominantly cytoplasmic in unligated form but translocates to the nucleus upon ligand-binding. Can also translocate to the nucleus in unligated form in the presence of RACK1.
  • Information by UniProt
  • Database links
  • FormThere are 2 isoforms produced by alternative splicing. Isoform 1 is also known as: AR-B; isoform 2 is known as AR-A or variant AR45.
  • Alternative names
    • AIS antibody
    • ANDR_HUMAN antibody
    • Androgen nuclear receptor variant 2 antibody
    • Androgen receptor (dihydrotestosterone receptor; testicular feminization; spinal and bulbar muscular atrophy; Kennedy disease) antibody
    • Androgen receptor antibody
    • androgen receptor splice variant 4b antibody
    • AR antibody
    • AR8 antibody
    • DHTR antibody
    • Dihydro testosterone receptor antibody
    • Dihydrotestosterone receptor (DHTR) antibody
    • Dihydrotestosterone receptor antibody
    • HUMARA antibody
    • HYSP1 antibody
    • KD antibody
    • Kennedy disease (KD) antibody
    • NR3C4 antibody
    • Nuclear receptor subfamily 3 group C member 4 (NR3C4) antibody
    • Nuclear receptor subfamily 3 group C member 4 antibody
    • SBMA antibody
    • SMAX1 antibody
    • Spinal and bulbar muscular atrophy (SBMA) antibody
    • Spinal and bulbar muscular atrophy antibody
    • Testicular Feminization (TFM) antibody
    • TFM antibody
    see all

Anti-Androgen Receptor antibody - ChIP Grade images

  • Immunohistochemistry (Formaline/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue labeling Androgen Receptor with ab3509 at 1/20 (right). Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 minutes. Tissues were blocked in 3% H2O2-methanol for 15 minutes at room temperature. A HRP-conjugated secondary antibody was used. Left - negative control.

References for Anti-Androgen Receptor antibody - ChIP Grade (ab3509)

This product has been referenced in:
  • Bhattacharya I  et al. A switch in Sertoli cell responsiveness to FSH may be responsible for robust onset of germ cell differentiation during prepubartal testicular maturation in rats. Am J Physiol Endocrinol Metab 303:E886-98 (2012). WB, ICC/IF ; Rat . Read more (PubMed: 22850685) »
  • Caraci F  et al. Neurotoxic properties of the anabolic androgenic steroids nandrolone and methandrostenolone in primary neuronal cultures. J Neurosci Res 89:592-600 (2011). WB . Read more (PubMed: 21290409) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Plethodontid Salamander Tissue sections (testes)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Unitrieve
Permeabilization No
Specification testes
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 75% · Temperature: 4°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 28 2015

Application Western blot
Loading amount 60 µg
Gel Running Conditions Reduced Denaturing (12)
Sample Human Cell lysate - whole cell (LNCaP)
Specification LNCaP
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Username

Abcam user community

Verified customer

Submitted Jan 13 2015

Application Western blot
Loading amount 60 µg
Gel Running Conditions Reduced Non-Denaturing (Native) (12)
Sample Mouse Cell lysate - whole cell (Mouse Testis)
Specification Mouse Testis
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Username

Dr. FRANCESCO ELIA MARINO

Verified customer

Submitted Mar 21 2014

Thank you for your reply.

I have had a look at ab9474 and the immunogen used to raise this antibody has been taken from residues 299-315 of human AR (SwissProt reference http://www.uniprot.org/uniprot/P10275). We do not have any information a...

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Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital informati...

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Thank you for your interest. It is very likely that ab9474 works in mouse but we have no data yet. According to Blast search, the homology between human and mouse AR is very high. http://www.uniprot.org/blast/uniprot/2011101050RXMELAA8 ...

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Thank you for providing the customer's full IP and western blotting protocols. Both protocols are very good and I have only a few questions I hope you can clarify: -has the customer tried WB with the RIPA buffer? The WB questionnaire mentions a t...

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Thank you for contacting us for technical support with ab3509. The fact that the customer does not have a lot number is not a problem, we are always happy to help our customers and will try hard to help them, even without a lot number. I would recomm...

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Thank you for the image you recently provided and for the customer's protocol details, it enabled me to understand the problem very rapidly. The customer's protocol is very good and similar to the one we recommend. The customer does not mention whether...

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This antibody is a rabbit polyclonal made using the recombinant full length protein from human. Being a polyclonal, it will therefore recognise a number of epitopes across the whole protein.

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