Anti-Androgen Receptor antibody - ChIP Grade (ab74272)

Overview

  • Product nameAnti-Androgen Receptor antibody - ChIP Grade
    See all Androgen Receptor primary antibodies
  • Description
    Rabbit polyclonal to Androgen Receptor - ChIP Grade
  • Tested applicationsSuitable for: ChIP, IP, WB, ICC/IF, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Androgen Receptor (N terminal). Sequence is propreitary.

  • Positive control
    • Human prostate carcinoma tissue. LnCap cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab74272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
IP Use at an assay dependent concentration. PubMed: 24466341
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 99 kDa.
ICC/IF Use at an assay dependent concentration.
IHC-P 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 mins, followed by cooling at RT for 20 mins.
IHC-Fr Use at an assay dependent concentration. PubMed: 21430025

Target

  • FunctionSteroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.
    Isoform 3 and isoform 4 lack the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.
  • Tissue specificityIsoform 2 is mainly expressed in heart and skeletal muscle (PubMed:15634333). Isoform 3 is expressed by basal and stromal cells of prostate (at protein level) (PubMed:19244107).
  • Involvement in diseaseAndrogen insensitivity syndrome
    Spinal and bulbar muscular atrophy X-linked 1
    Defects in AR may play a role in metastatic prostate cancer. The mutated receptor stimulates prostate growth and metastases development despite of androgen ablation. This treatment can reduce primary and metastatic lesions probably by inducing apoptosis of tumor cells when they express the wild-type receptor.
    Androgen insensitivity, partial
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. In the presence of bound steroid the ligand-binding domain interacts with the N-terminal modulating domain, and thereby activates AR transcription factor activity. Agonist binding is required for dimerization and binding to target DNA. The transcription factor activity of the complex formed by ligand-activated AR and DNA is modulated by interactions with coactivator and corepressor proteins. Interaction with RANBP9 is mediated by both the N-terminal domain and the DNA-binding domain. Interaction with EFCAB6/DJBP is mediated by the DNA-binding domain.
  • Post-translational
    modifications
    Sumoylated on Lys-388 (major) and Lys-521. Ubiquitinated. Deubiquitinated by USP26. 'Lys-6' and 'Lys-27'-linked polyubiquitination by RNF6 modulates AR transcriptional activity and specificity.
    Phosphorylated in prostate cancer cells in response to several growth factors including EGF. Phosphorylation is induced by c-Src kinase (CSK). Tyr-535 is one of the major phosphorylation sites and an increase in phosphorylation and Src kinase activity is associated with prostate cancer progression. Phosphorylation by TNK2 enhances the DNA-binding and transcriptional activity and may be responsible for androgen-independent progression of prostate cancer. Phosphorylation at Ser-83 by CDK9 regulates AR promoter selectivity and cell growth. Phosphorylation by PAK6 leads to AR-mediated transcription inhibition.
    Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
  • Cellular localizationNucleus. Cytoplasm. Predominantly cytoplasmic in unligated form but translocates to the nucleus upon ligand-binding. Can also translocate to the nucleus in unligated form in the presence of RACK1.
  • Information by UniProt
  • Database links
  • FormThere are 2 isoforms produced by alternative splicing. Isoform 1 is also known as: AR-B; isoform 2 is known as AR-A or variant AR45.
  • Alternative names
    • AIS antibody
    • ANDR_HUMAN antibody
    • Androgen nuclear receptor variant 2 antibody
    • Androgen receptor (dihydrotestosterone receptor; testicular feminization; spinal and bulbar muscular atrophy; Kennedy disease) antibody
    • Androgen receptor antibody
    • androgen receptor splice variant 4b antibody
    • AR antibody
    • AR8 antibody
    • DHTR antibody
    • Dihydro testosterone receptor antibody
    • Dihydrotestosterone receptor (DHTR) antibody
    • Dihydrotestosterone receptor antibody
    • HUMARA antibody
    • HYSP1 antibody
    • KD antibody
    • Kennedy disease (KD) antibody
    • NR3C4 antibody
    • Nuclear receptor subfamily 3 group C member 4 (NR3C4) antibody
    • Nuclear receptor subfamily 3 group C member 4 antibody
    • SBMA antibody
    • SMAX1 antibody
    • Spinal and bulbar muscular atrophy (SBMA) antibody
    • Spinal and bulbar muscular atrophy antibody
    • Testicular Feminization (TFM) antibody
    • TFM antibody
    see all

Anti-Androgen Receptor antibody - ChIP Grade images

  • ab74272 staining Androgen Receptor in Mouse neuroblastoma cells by Immunocytochemistry/Immunofluorescence. Cells were fixed in formaldehyde and permeabilized in 0.2% Triton X-100 prior to blocking in 3% BSA for 1 hour. The primary antibody was diluted 1/200 and incubated with the sample for 12 hours at 4°C. The secondary antibody was Alexa Fluor® 594-conjugated Goat anti-Rabbit polyclonal, diluted 1/400.

    See Abreview

  • Formalij fixed/paraffin embedded human prostate carcinoma stained with 1/200 ab74272 (30mins, RT).

  • ab74272 used in ChIP application.Whole cell lysate from human LNCaP cells was used.Cross linking step (X-ChIP) for 10 minutes in 1% formaldehyde.Taqman detection step.Positive control was PSA enhancer.Negative control region, no antibody control, and in the absence of ligand.25 µl undiluted ab74272 per IP was used.

    See Abreview

References for Anti-Androgen Receptor antibody - ChIP Grade (ab74272)

This product has been referenced in:
  • Ma Q  et al. A Novel Missense Mutation in USP26 Gene Is Associated With Nonobstructive Azoospermia. Reprod Sci 23:1434-41 (2016). Read more (PubMed: 27089915) »
  • Venant H  et al. The Sphingosine Kinase 2 Inhibitor ABC294640 Reduces the Growth of Prostate Cancer Cells and Results in Accumulation of Dihydroceramides In Vitro and In Vivo. Mol Cancer Ther 14:2744-52 (2015). Read more (PubMed: 26494858) »

See all 14 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (LNCaP - prostate cancer cells)
Permeabilization Yes - 0.2% Triton X-100
Specification LNCaP - prostate cancer cells
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

Submitted Jun 28 2016

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 25°C
Sample Mouse Tissue sections (adult mouse testis)
Specification adult mouse testis
Permeabilization No
Fixative 4% PFA
Username

Dr. Qing Wen

Verified customer

Submitted Dec 31 2014

The immunogen sequence is considered confidential by the laboratory that produces it. We only know that it is located within aa 180-320 of accession P10275, http://www.uniprot.org/uniprot/P10275.

ChIP

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Detection step Semiquantitative PCR
Sample Mouse Tissue lysate - whole (epididymis)
Specification epididymis
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Username

Ms. JaeEun Lee

Verified customer

Submitted May 13 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (epididymis)
Specification epididymis
Fixative Bouin's solution
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 30 2013

The peptide for ab74272 is commercially not available. I am sorry we cannot provide this.
The peptide ab45603 is suitable to be used with ab19066, not with ab74272.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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Thank you for taking time to provide these additional details and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us w...

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Thank you for providing this further information.

I appreciate your cooperation and understand your concerns. It is regrettable the results have not been succesful.

In order to help with our investigation of this case before d...

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Thank you for contacting us. Unfortunately, the lab has confirmed that we do not have any remaining material from any of these lots. Please let me know if there is anything else I can do to assist you further.

1-10 of 18 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"