Anti-Androgen Receptor antibody [EP670Y] (ab52615)

Overview

  • Product nameAnti-Androgen Receptor antibody [EP670Y]
    See all Androgen Receptor primary antibodies
  • Description
    Rabbit monoclonal [EP670Y] to Androgen Receptor
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Androgen Receptor aa 900 to the C-terminus (C terminal). The exact sequence is proprietary.
    (Peptide available as ab174742)

  • Positive control
    • WB: LnCaP and PC-3 cell lysates and mouse and rat testis tissue lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab52615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 99 kDa.Can be blocked with Androgen Receptor peptide (ab174742).
  • Application notesIs unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • FunctionSteroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.
      Isoform 3 and isoform 4 lack the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.
    • Tissue specificityIsoform 2 is mainly expressed in heart and skeletal muscle (PubMed:15634333). Isoform 3 is expressed by basal and stromal cells of prostate (at protein level) (PubMed:19244107).
    • Involvement in diseaseAndrogen insensitivity syndrome
      Spinal and bulbar muscular atrophy X-linked 1
      Defects in AR may play a role in metastatic prostate cancer. The mutated receptor stimulates prostate growth and metastases development despite of androgen ablation. This treatment can reduce primary and metastatic lesions probably by inducing apoptosis of tumor cells when they express the wild-type receptor.
      Androgen insensitivity, partial
    • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. In the presence of bound steroid the ligand-binding domain interacts with the N-terminal modulating domain, and thereby activates AR transcription factor activity. Agonist binding is required for dimerization and binding to target DNA. The transcription factor activity of the complex formed by ligand-activated AR and DNA is modulated by interactions with coactivator and corepressor proteins. Interaction with RANBP9 is mediated by both the N-terminal domain and the DNA-binding domain. Interaction with EFCAB6/DJBP is mediated by the DNA-binding domain.
    • Post-translational
      modifications
      Sumoylated on Lys-388 (major) and Lys-521. Ubiquitinated. Deubiquitinated by USP26. 'Lys-6' and 'Lys-27'-linked polyubiquitination by RNF6 modulates AR transcriptional activity and specificity.
      Phosphorylated in prostate cancer cells in response to several growth factors including EGF. Phosphorylation is induced by c-Src kinase (CSK). Tyr-535 is one of the major phosphorylation sites and an increase in phosphorylation and Src kinase activity is associated with prostate cancer progression. Phosphorylation by TNK2 enhances the DNA-binding and transcriptional activity and may be responsible for androgen-independent progression of prostate cancer. Phosphorylation at Ser-83 by CDK9 regulates AR promoter selectivity and cell growth. Phosphorylation by PAK6 leads to AR-mediated transcription inhibition.
      Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
    • Cellular localizationNucleus. Cytoplasm. Predominantly cytoplasmic in unligated form but translocates to the nucleus upon ligand-binding. Can also translocate to the nucleus in unligated form in the presence of RACK1.
    • Information by UniProt
    • Database links
    • FormThere are 2 isoforms produced by alternative splicing. Isoform 1 is also known as: AR-B; isoform 2 is known as AR-A or variant AR45.
    • Alternative names
      • AIS antibody
      • ANDR_HUMAN antibody
      • Androgen nuclear receptor variant 2 antibody
      • Androgen receptor (dihydrotestosterone receptor; testicular feminization; spinal and bulbar muscular atrophy; Kennedy disease) antibody
      • Androgen receptor antibody
      • androgen receptor splice variant 4b antibody
      • AR antibody
      • AR8 antibody
      • DHTR antibody
      • Dihydro testosterone receptor antibody
      • Dihydrotestosterone receptor (DHTR) antibody
      • Dihydrotestosterone receptor antibody
      • HUMARA antibody
      • HYSP1 antibody
      • KD antibody
      • Kennedy disease (KD) antibody
      • NR3C4 antibody
      • Nuclear receptor subfamily 3 group C member 4 (NR3C4) antibody
      • Nuclear receptor subfamily 3 group C member 4 antibody
      • SBMA antibody
      • SMAX1 antibody
      • Spinal and bulbar muscular atrophy (SBMA) antibody
      • Spinal and bulbar muscular atrophy antibody
      • Testicular Feminization (TFM) antibody
      • TFM antibody
      see all

    Anti-Androgen Receptor antibody [EP670Y] images

    • All lanes : Anti-Androgen Receptor antibody [EP670Y] (ab52615) at 1/2000 dilution (purified)

      Lane 1 : Mouse testis tissue lysate
      Lane 2 : Rat testis tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size : 99 kDa
      Observed band size : 110 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Anti-Androgen Receptor antibody [EP670Y] (ab52615) at 1/1000 dilution (purified) + PC-3 whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size : 99 kDa
      Observed band size : 110 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Anti-Androgen Receptor antibody [EP670Y] (ab52615) at 1/10000 dilution (unpurified) + LnCaP cell lysate at 10 µg

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 99 kDa
      Observed band size : 99 kDa

    References for Anti-Androgen Receptor antibody [EP670Y] (ab52615)

    This product has been referenced in:
    • Clinard CT  et al. Winning agonistic encounters increases testosterone and androgen receptor expression in Syrian hamsters. Horm Behav 86:27-35 (2016). Read more (PubMed: 27619945) »
    • Cochrane DR  et al. Role of the Androgen Receptor in Breast Cancer and Preclinical Analysis of Enzalutamide. Breast Cancer Res 16:R7 (2014). WB ; Human . Read more (PubMed: 24451109) »

    See all 12 Publications for this product

    Product Wall

    Thank you very much for your interest our products.


    To our knowledge, ab133273 not been tested in IHC-FrFl (Immunohistochemistry - Free Floating).

    However by participating in our AbTrial program you can now use our products in...

    Read More
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
    Antigen retrieval step None
    Sample Hamster Tissue sections (neuronal (amygdala)cells)
    Specification neuronal (amygdala)cells
    Permeabilization No
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Mar 26 2014

    Thank you for your inquiry.

    I can confirm that in our hands ab52615 (1852-1) is not working in IHC, ICC and IP. Therefore this antibody is not covered by our guarantee since we are not convinced that ab52615 is suitable for these applications...

    Read More
    Application Immunocytochemistry
    Sample Human Cell (Human AR receptor construct expressed in live dros)
    Specification Human AR receptor construct expressed in live dros
    Fixative Paraformaldehyde
    Permeabilization Yes - PBS-Triton(0.3%)
    Blocking step PBST/10%Goat serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 26 2012

    Thank you for contacting us and your interest in our products.

    As discussed over the phone, we do not provide tester samples of our antibodies simply due to the logistics that would be involved in this. With over 70,000 products it simply isn...

    Read More

    Thank you for your interest. It is very likely that ab9474 works in mouse but we have no data yet. According to Blast search, the homology between human and mouse AR is very high. http://www.uniprot.org/blast/uniprot/2011101050RXMELAA8 ...

    Read More

    Thank you for contacting Abcam.
    The peptide maps between aa ********** on the C-terminus of human Androgen receptor (UniProt: P10275).
    Please let me know if there is anything else I can help with you.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"