Androstenedione ELISA Kit (ab108672)
- Product nameAndrostenedione ELISA KitSee all Androstenedione kits ...
- Detection methodColorimetric
Intra-assay Sample n Mean SD CV% Control Sera 60 <=10%
- Tests1 x 96 test
- Sample typeSerum, Plasma
- Assay typeCompetitive
- Sensitivity= 0.01 ng/ml
- Range0.1 ng/ml - 10 ng/ml
- Assay durationMultiple steps standard assay
- Product overview
Abcam’s Androstenedione in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Androstenedione in serum and plasma.
A 96-well plate has been precoated with anti-Androstenedione IgG. Samples and the Androstenedione-HRP conjugate are added to the wells, where Androstenedione in the sample competes with the added Androstenedione-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Androstenedione in the sample and the intensity is measured at 450 nm.
- Tested applicationsCompetitive ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Washing Solution 1 x 50ml Androstenedione Control 1 x 1ml Androstenedione Standard 0 – 0 ng/mL 1 x 1ml Androstenedione Standard 1 – 0.1 ng/mL 1 x 1ml Androstenedione Standard 2 – 0.4 ng/mL 1 x 1ml Androstenedione Standard 3 – 1.2 ng/mL 1 x 1ml Androstenedione Standard 4 – 4.0 ng/mL 1 x 1ml Androstenedione Standard 5 – 10.0 ng/mL 1 x 1ml Androstenedione-HRP Conjugate 1 x 21ml Anti-Androstenedione IgG Coated Microplate (12 x 8 wells) 1 unit Cover foils 1 unit Stop Solution 1 x 15ml Strip holder 1 unit TMB Substrate Solution 1 x 15ml
- Research Areas
- 4 androstenedione
Our Abpromise guarantee covers the use of ab108672 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Androstenedione ELISA Kit (ab108672)
ab108672 has not yet been referenced specifically in any publications.