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Abcam’s Androstenedione in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Androstenedione in serum and plasma.
A 96-well plate has been precoated with anti-Androstenedione IgG. Samples and the Androstenedione-HRP conjugate are added to the wells, where Androstenedione in the sample competes with the added Androstenedione-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Androstenedione in the sample and the intensity is measured at 450 nm.
|Components||1 x 96 tests|
|10X Washing Solution||1 x 50ml|
|Androstenedione Control||1 x 1ml|
|Androstenedione Standard 0 – 0 ng/mL||1 x 1ml|
|Androstenedione Standard 1 – 0.1 ng/mL||1 x 1ml|
|Androstenedione Standard 2 – 0.4 ng/mL||1 x 1ml|
|Androstenedione Standard 3 – 1.2 ng/mL||1 x 1ml|
|Androstenedione Standard 4 – 4.0 ng/mL||1 x 1ml|
|Androstenedione Standard 5 – 10.0 ng/mL||1 x 1ml|
|Androstenedione-HRP Conjugate||1 x 21ml|
|Anti-Androstenedione IgG Coated Microplate (12 x 8 wells)||1 unit|
|Cover foils||1 unit|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab108672 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Competitive ELISA||Use at an assay dependent dilution.|
ab108672 has not yet been referenced specifically in any publications.