Overview

  • Product name
    Androstenedione ELISA Kit
    See all Androstenedione kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Serum 16 <=8.5%
    Inter-assay
    Sample n Mean SD CV%
    Saliva 10 <=11%
  • Sample type
    Saliva
  • Assay type
    Competitive
  • Sensitivity
    5 pg/ml
  • Range
    20 pg/ml - 1000 pg/ml
  • Recovery

    102.6 %

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Androstenedione in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Androstenedione in saliva.

    A 96-well plate has been precoated with anti-Androstenedione antibodies. Samples and the Androstenedione-HRP conjugate are added to the wells, where Androstenedione in the sample competes with the added Androstenedione-HRP conjugate for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Androstenedione in the sample and the intensity is measured at 450 nm.

  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    50X Washing Solution 1 x 20ml
    Androstenedione HRP Conjugate 1 x 1ml
    Androstenedione Standard 0 - 0 pg/mL 1 x 1ml
    Androstenedione Standard 1 - 20 pg/mL 1 x 1ml
    Androstenedione Standard 2 - 100 pg/mL 1 x 1ml
    Androstenedione Standard 3 - 400 pg/mL 1 x 1ml
    Androstenedione Standard 4 - 1,000 pg/mL 1 x 1ml
    Anti-Androstenedione IgG Coated Microplate (12 x 8 wells) 1 unit
    Control A 1 vial
    Control B 1 vial
    Cover Foil 1 unit
    Incubation Buffer 1 x 30ml
    Stop Solution 1 x 15ml
    Strip holder 1 unit
    TMB Substrate Solution 1 x 15ml
  • Research areas
  • Alternative names
    • 4 androstenedione

Applications

Our Abpromise guarantee covers the use of ab178609 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab178609.

Protocols

References

ab178609 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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