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Abcam’s Androstenedione in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Androstenedione in saliva.
A 96-well plate has been precoated with anti-Androstenedione antibodies. Samples and the Androstenedione-HRP conjugate are added to the wells, where Androstenedione in the sample competes with the added Androstenedione-HRP conjugate for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Androstenedione in the sample and the intensity is measured at 450 nm.
|Components||1 x 96 tests|
|50X Washing Solution||1 x 20ml|
|Androstenedione HRP Conjugate||1 x 1ml|
|Androstenedione Standard 0 - 0 pg/mL||1 x 1ml|
|Androstenedione Standard 1 - 20 pg/mL||1 x 1ml|
|Androstenedione Standard 2 - 100 pg/mL||1 x 1ml|
|Androstenedione Standard 3 - 400 pg/mL||1 x 1ml|
|Androstenedione Standard 4 - 1,000 pg/mL||1 x 1ml|
|Anti-Androstenedione IgG Coated Microplate (12 x 8 wells)||1 unit|
|Control A||1 vial|
|Control B||1 vial|
|Cover Foil||1 unit|
|Incubation Buffer||1 x 30ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab178609 in the following tested applications.
|Competitive ELISA||Use at an assay dependent concentration.|
ab178609 has not yet been referenced specifically in any publications.