Overview

  • Product nameAngiogenin (ANG) Human ELISA Kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 1.5 pg/ml
  • Range
    1.64 pg/ml - 400 pg/ml
  • Recovery

    94.7 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.37 85% - 104%
    Serum 95.95 82% - 105%
    Plasma 93.49 83% - 104%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Angiogenin (ANG) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Angiogenin in serum, plasma and cell culture supernates.

    This assay employs an antibody specific for Human Angiogenin coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiogenin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Angiogenin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiogenin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceAngiogenin is a member of the ribonuclease superfamily with approximately 35% amino acid sequence identity with pancreatic RNase. Like other members of the ribonuclease superfamily, angiogenin is a cytotoxic agent that can eliminate cellular protein synthesis. Angiogenin causes inhibition of protein synthesis by functioning as a cytotoxic tRNA-specific RNase. Angiogenesis is the formation of blood vessels or capillaries from existing blood vessels due to specific signals. Angiogenin induces the growth of new blood vessels. It stimulates capillary and umbilical vein endothelial cells to produce diacylglycerol and secrete prostacyclin by phospholipase activation. Angiogenin exhibits both angiogenic and non-angiogenic activities and is involved in endothelial cell migration, proliferation, and differentiation. It is produced by a variety of tumor and normal cell types. Cells that express angiogenin include vascular endothelial and smooth muscle cells, fibroblasts, normal colonic epithelium, normal peripheral blood lymphocytes, lung and colonic epithelial tumor cell lines, and primary gastrointestinal adenocarcinomas. Angiogenin is also present in normal human plasma.
  • Alternative names
    • ALS9
    • ANG
    • ANG 1
    • ANG I
    • ANG1
    • ANGI
    • Angiogenin ribonuclease RNase A family 5
    • Epididymis luminal protein 168
    • HEL168
    • MGC22466
    • MGC71966
    • Ribonuclease 5
    • Ribonuclease RNase A Family 5
    • Ribonuclease5
    • RNASE 5
    • RNASE4
    • RNASE5
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab99970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Angiogenin (ANG) Human ELISA Kit images

  • Representative standard curve using ab99970.

  • Representative standard curve using ab99970.

Protocols

References for Angiogenin (ANG) Human ELISA Kit (ab99970)

This product has been referenced in:
  • Miyake M  et al. Angiogenin promotes tumoral growth and angiogenesis by regulating matrix metallopeptidase-2 expression via the ERK1/2 pathway. Oncogene N/A:N/A (2014). Read more (PubMed: 24561529) »

See 1 Publication for this product

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