Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab95389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 mg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 17 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    May function as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically hydrolyzing cellular tRNAs. Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo.
  • Tissue specificity
    Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons.
  • Involvement in disease
    Defects in ANG are the cause of susceptibility to amyotrophic lateral sclerosis type 9 (ALS9) [MIM:611895]. ALS is a degenerative disorder of motor neurons in the cortex, brain stem and spinal cord. ALS is characterized by muscular weakness and atrophy.
  • Sequence similarities
    Belongs to the pancreatic ribonuclease family.
  • Developmental stage
    Low level expression in the developing fetus, increased in the neonate, and maximal in the adult.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALS9 antibody
    • AMYOTROPHIC LATERAL SCLEROSIS antibody
    • ANG 1 antibody
    • ANG antibody
    • ANG I antibody
    • ANG1 antibody
    • ANGI antibody
    • ANGI_HUMAN antibody
    • Angiogenin antibody
    • Angiogenin ribonuclease RNase A family, 5 antibody
    • Epididymis luminal protein 168 antibody
    • HEL168 antibody
    • MGC22466 antibody
    • MGC71966 antibody
    • Ribonuclease 5 antibody
    • Ribonuclease RNase A Family 5 antibody
    • RNase 5 antibody
    • RNASE4 antibody
    • RNASE5 antibody
    see all

Images

  • All lanes : Anti-Angiogenin antibody (ab95389) at 1 µg/ml

    Lane 1 : Lung (Human) Tissue Lysate
    Lane 2 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 17 kDa
    Observed band size : 16 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 125 kDa,160 kDa,23 kDa,50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • ICC/IF image of ab95389 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab95389 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab95389 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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