The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 58 kDa).
FunctionBinds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development.
Western blot - Anti-Angiopoietin 1 antibody (ab118380)
Lane 1 : Anti-Angiopoietin 1 antibody (ab118380) at 1 µg/ml Lane 2 : Anti-Angiopoietin 1 antibody (ab118380) at 1 mg/ml Lane 3 : Anti-DYKDDDDK Tag antibody at 1/1000 dilution
Lane 1 : HEK293T cell lysate transfected with Angiopoietin 1 (myc and DYKDDDDK tagged) at 10 µg Lane 2 : HEK293T cell lysate mock transfected Lane 3 : HEK293T cell lysate transfected with Angiopoietin 1 (myc and DYKDDDDK tagged) at 10 µg
developed using the ECL technique
Predicted band size : 58 kDa
References for Anti-Angiopoietin 1 antibody (ab118380)
has not yet been referenced specifically in any publications.
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