Overview

  • Product name
    Anti-Angiopoietin 1 antibody
    See all Angiopoietin 1 primary antibodies
  • Description
    Rabbit polyclonal to Angiopoietin 1
  • Tested applications
    Suitable for: IHC-Fr, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A 15 amino acid synthetic peptide from near the N terminal residues of Human Angiopoietin 1 (NM_001146.3).

  • Positive control
    • Heart lysates; heart tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab94684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
WB
IHC-P
  • Application notes
    IHC-P: 1/50 - 1/20.
    WB: 1/200 - 1/500. Predicted molecular weight: 58 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development.
    • Sequence similarities
      Contains 1 fibrinogen C-terminal domain.
    • Post-translational
      modifications
      Glycosylated.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • AGP 1 antibody
      • AGP1 antibody
      • AGPT antibody
      • ANG 1 antibody
      • ANG antibody
      • ANG-1 antibody
      • ANG1 antibody
      • Angiopoietin-1 antibody
      • Angiopoietin1 antibody
      • ANGP1_HUMAN antibody
      • ANGPT 1 antibody
      • Angpt1 antibody
      • KIAA0003 antibody
      • OTTHUMP00000227592 antibody
      • OTTHUMP00000227599 antibody
      • OTTHUMP00000227600 antibody
      see all

    Images

    • ab94684, at 1/100 dilution, staining Angiopoietin 1 in formalin fixed, paraffin embedded human heart tissue by Immunohistochemistry.
    • Anti-Angiopoietin 1 antibody (ab94684) at 1/200 dilution + human heart lysate

      Predicted band size : 58 kDa

    References

    This product has been referenced in:

    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (lung)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native)
    Loading amount
    30 µg
    Specification
    lung
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Oct 05 2015

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Sample
    Mouse Tissue sections (liver)
    Specification
    liver
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jan 08 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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