Anti-Angiopoietin 2 antibody (ab64015)

Overview

  • Product nameAnti-Angiopoietin 2 antibody
    See all Angiopoietin 2 primary antibodies
  • Description
    Rabbit polyclonal to Angiopoietin 2
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig, Rhesus monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human Angiopoietin 2.

    (Peptide available as ab99482.)

  • Positive control
    • This antibogy gave a positive signal in the following human tissue lysates: Ovary; Cervix; Placenta; Lung.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab64015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).Can be blocked with Human Angiopoietin 2 peptide (ab99482).

Target

Anti-Angiopoietin 2 antibody images

  • All lanes : Anti-Angiopoietin 2 antibody (ab64015) at 1 µg/ml

    Lane 1 : Ovary (Human) Tissue Lysate - adult normal tissue (ab30222)
    Lane 2 : Cervix (Human) Whole Cell Lysate - adult normal tissue (ab29226)
    Lane 3 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745)
    Lane 4 : Lung (Human) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 57 kDa
    Observed band size : 57 kDa
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References for Anti-Angiopoietin 2 antibody (ab64015)

ab64015 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.
I have aligned the human angiopoietin 1 and 2 proteins and the overall sequence homology is only 61%.
I would recommend ab64015, since the immunogen does not align well with Ang1 (63% homology)
http://www.abcam....

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Thank you for contacting us. Antibody ab65835 is our most popular Ang-2 antibody. It does not require an IP to obtain good blots. The datasheet includes an image of a blot of a rat brain lysate that was loaded onto the gel after homogenization and ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"