Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer0.02 K Phosphate, 0.15M NaCl, 0.1% Sodium Azide, pH7.2
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab8452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
IHC-Fr Use at an assay dependent concentration. PubMed: 19553662Use at an assay dependent dilution (PMID 19553662).
WB 1/500. Can be blocked with Mouse Angiopoietin 2 peptide (ab9077). There is reaction with serum in the cell supernatants, which results in strong background and makes it difficult to see the angiopoietins in cell supernatants. However when precipitated (using soluble Tie2) the signals are very good and strong.

Target

  • FunctionCan induce tyrosine phosphorylation of TIE2. Binds to TIE2 receptor and counteracts blood vessel maturation/stability mediated by angiopoietin-1. Its function may be context-dependent. In the absence of angiogenic inducers, such as VEGF, ANG2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
  • Sequence similaritiesContains 1 fibrinogen C-terminal domain.
  • DomainThe Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • AGPT 2 antibody
    • Agpt2 antibody
    • ANG 2 antibody
    • ANG-2 antibody
    • ANG2 antibody
    • Angiopoietin 2a antibody
    • Angiopoietin 2B antibody
    • Angiopoietin-2 antibody
    • Angiopoietin2 antibody
    • ANGP2_HUMAN antibody
    • ANGPT 2 antibody
    • Angpt2 antibody
    • Tie2 ligand antibody
    see all

Anti-Angiopoietin 2 antibody images

  • This image is courtesy of Marion Scharpfenecker 2002

    Supernatants of mouse angiopoietin-expressing endothelial cells. Soluble Tie 2 was used to precipitate the angiopoietins to reduce background.

    Lane 1 - mock
    Lane 2 - mouse angiopoietin-2 (clone 2-9) expressing cells
    Lane 3 - mouse angiopoietin-1 (clone 1-15) expressing cells
    Lane 4 - mouse angiopoietin-1 (clone 1-8) expressing cells
    Lane 5 - wt

    Supernatants of mouse angiopoietin-expressing endothelial cells. Soluble Tie 2 was used to precipitate the angiopoietins to reduce background.

    Lane 1 - mock
    Lane 2 - mouse angiopoietin-2 (clone 2-9) expressing cells
    Lane 3 - mouse angiopoietin-1 (clone 1-15) expressing cells
    Lane 4 - mouse angiopoietin-1 (clone 1-8) expressing cells
    Lane 5 - wt

  • ICC/IF image of ab8452 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8452, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of murine brain coronal sections, staining Angiopoietin 2 with ab8452 at 1/500 dilution. Sections were taken from control mice (left) or mice subjected to middle cerebral artery occlusion (right).

References for Anti-Angiopoietin 2 antibody (ab8452)

This product has been referenced in:
  • Mofarrahi M  et al. Angiopoietin-1 enhances skeletal muscle regeneration in mice. Am J Physiol Regul Integr Comp Physiol 308:R576-89 (2015). IHC-Fr ; Mouse . Read more (PubMed: 25608750) »
  • Chu X  et al. Multiple microvascular alterations in pancreatic islets and neuroendocrine tumors of a Men1 mouse model. Am J Pathol 182:2355-67 (2013). IHC ; Mouse . Read more (PubMed: 23583653) »

See all 7 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (mouse kidney)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization Yes - 0.5% Triton X100
Specification mouse kidney
Blocking step 2.5% BSA / 2.5% donkey serum as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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Submitted Jun 03 2016

Application Western blot
Sample Mouse Tissue lysate - whole (lung)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 30 µg
Treatment OVA I.P, I.N
Specification lung
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Oct 05 2015

Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Denaturing (10%gel)
Sample Mouse Tissue lysate - whole (lung)
Specification lung
Treatment OVA-induce
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 38°C
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Submitted Dec 03 2012

Thank you very much for your reply.

I will forward your request to my colleagues in our Hong Kong office, and someone will be in touch with you promptly.

Have a nice day!

Thank you very much for your valuable review.
I'm writing to follow-up with you, as the results were below average and we do guarantee this antibody to work in Western blot with mouse samples. I'm sorry to see that there were several non-specific ...

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Thank you for your enquiry. The Tie2 precipitation experiment was performed by an outside collaborator, and we do not have any further information at hand regarding this. I have tried to email the collaborator to see if we can obtain this infor...

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Thank you for your enquiry. ab8451 is specific for mouse Angiopoietin 1. ab8452 cross-reacts with human (reacts with Ang-2 and weakly with Ang-1) and mouse (specific for mAng-2). There is little sequence homology between the two epitopes of 1 vers...

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All the information available for this product is listed on the on-line datasheet (price, datasheet, publication, suitability, cross-reactivity). Angiopoietin-2 is a naturally occurring antagonist of angiopoietin-1. Western blot analysis showed tha...

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This antibody has never been tested in IHC on mouse tissue sections, therefore we can't guarantee results.

We have not tested this antibody for reactivity towards the rat ang-2. We would be very interested to hear the results.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"