Anti-Angiopoietin 2 antibody [MM0020-1F29] (ab56301)


  • Product nameAnti-Angiopoietin 2 antibody [MM0020-1F29]
    See all Angiopoietin 2 primary antibodies
  • Description
    Mouse monoclonal [MM0020-1F29] to Angiopoietin 2
  • SpecificityThis antibody recognizes Angiopoietin 2 in western blot without cross reactivity with Angiopoietin 1.
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant purified full length protein (Human).

  • Positive control
    • Human endothelial cell lysate.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThe IgG fraction of the tissue culture supernatant was purified and then lyophilized from 0.2 µm filtered PBS solution.
  • ClonalityMonoclonal
  • Clone numberMM0020-1F29
  • IsotypeIgG2
  • Research areas


Our Abpromise guarantee covers the use of ab56301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/200.
WB 1/400 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 57 kDa).


  • FunctionCan induce tyrosine phosphorylation of TIE2. Binds to TIE2 receptor and counteracts blood vessel maturation/stability mediated by angiopoietin-1. Its function may be context-dependent. In the absence of angiogenic inducers, such as VEGF, ANG2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
  • Sequence similaritiesContains 1 fibrinogen C-terminal domain.
  • DomainThe Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • AGPT 2 antibody
    • Agpt2 antibody
    • ANG 2 antibody
    • ANG-2 antibody
    • ANG2 antibody
    • Angiopoietin 2a antibody
    • Angiopoietin 2B antibody
    • Angiopoietin-2 antibody
    • Angiopoietin2 antibody
    • ANGP2_HUMAN antibody
    • ANGPT 2 antibody
    • Angpt2 antibody
    • Tie2 ligand antibody
    see all

Anti-Angiopoietin 2 antibody [MM0020-1F29] images

  • ab56301 at 1/200 dilution, staining Angiopoietin 2 in human placental tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).

  • Anti-Angiopoietin 2 antibody [MM0020-1F29] (ab56301) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 57 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 47 kDa,65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

References for Anti-Angiopoietin 2 antibody [MM0020-1F29] (ab56301)

ab56301 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact ...

Read More

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

Read More

Thank you for your reply.
I have aligned the human angiopoietin 1 and 2 proteins and the overall sequence homology is only 61%.
I would recommend ab64015, since the immunogen does not align well with Ang1 (63% homology)

Read More