Anti-Angiopoietin 2 antibody [MM0020-1F29] (ab56301)


  • Product nameAnti-Angiopoietin 2 antibody [MM0020-1F29]
    See all Angiopoietin 2 primary antibodies
  • Description
    Mouse monoclonal [MM0020-1F29] to Angiopoietin 2
  • SpecificityThis antibody recognizes Angiopoietin 2 in western blot without cross reactivity with Angiopoietin 1.
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant purified full length protein (Human).

  • Positive control
    • Human endothelial cell lysate.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThe IgG fraction of the tissue culture supernatant was purified and then lyophilized from 0.2 µm filtered PBS solution.
  • ClonalityMonoclonal
  • Clone numberMM0020-1F29
  • IsotypeIgG2
  • Research areas


Our Abpromise guarantee covers the use of ab56301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/200.
WB 1/400 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 57 kDa).


  • FunctionCan induce tyrosine phosphorylation of TIE2. Binds to TIE2 receptor and counteracts blood vessel maturation/stability mediated by angiopoietin-1. Its function may be context-dependent. In the absence of angiogenic inducers, such as VEGF, ANG2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
  • Sequence similaritiesContains 1 fibrinogen C-terminal domain.
  • DomainThe Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • AGPT 2 antibody
    • Agpt2 antibody
    • ANG 2 antibody
    • ANG-2 antibody
    • ANG2 antibody
    • Angiopoietin 2a antibody
    • Angiopoietin 2B antibody
    • Angiopoietin-2 antibody
    • Angiopoietin2 antibody
    • ANGP2_HUMAN antibody
    • ANGPT 2 antibody
    • Angpt2 antibody
    • Tie2 ligand antibody
    see all

Anti-Angiopoietin 2 antibody [MM0020-1F29] images

  • ab56301 at 1/200 dilution, staining Angiopoietin 2 in human placental tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).

  • Anti-Angiopoietin 2 antibody [MM0020-1F29] (ab56301) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 57 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 47 kDa,65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

References for Anti-Angiopoietin 2 antibody [MM0020-1F29] (ab56301)

ab56301 has not yet been referenced specifically in any publications.

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Thank you for your reply.
I have aligned the human angiopoietin 1 and 2 proteins and the overall sequence homology is only 61%.
I would recommend ab64015, since the immunogen does not align well with Ang1 (63% homology)

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