Anti-Angiopoietin 2 antibody [MM0020-1F29] (ab56301)

Overview

  • Product name
    Anti-Angiopoietin 2 antibody [MM0020-1F29]
    See all Angiopoietin 2 primary antibodies
  • Description
    Mouse monoclonal [MM0020-1F29] to Angiopoietin 2
  • Specificity
    This antibody recognizes Angiopoietin 2 in western blot without cross reactivity with Angiopoietin 1.
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant purified full length protein (Human).

  • Positive control
    • Human endothelial cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    The IgG fraction of the tissue culture supernatant was purified and then lyophilized from 0.2 µm filtered PBS solution.
  • Clonality
    Monoclonal
  • Clone number
    MM0020-1F29
  • Isotype
    IgG2
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab56301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/200.
WB 1/400 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 57 kDa).

Target

  • Function
    Can induce tyrosine phosphorylation of TIE2. Binds to TIE2 receptor and counteracts blood vessel maturation/stability mediated by angiopoietin-1. Its function may be context-dependent. In the absence of angiogenic inducers, such as VEGF, ANG2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
  • Sequence similarities
    Contains 1 fibrinogen C-terminal domain.
  • Domain
    The Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • AGPT 2 antibody
    • Agpt2 antibody
    • ANG 2 antibody
    • ANG-2 antibody
    • ANG2 antibody
    • Angiopoietin 2a antibody
    • Angiopoietin 2B antibody
    • Angiopoietin-2 antibody
    • Angiopoietin2 antibody
    • ANGP2_HUMAN antibody
    • ANGPT 2 antibody
    • Angpt2 antibody
    • Tie2 ligand antibody
    see all

Images

  • ab56301 at 1/200 dilution, staining Angiopoietin 2 in human placental tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).

  • Anti-Angiopoietin 2 antibody [MM0020-1F29] (ab56301) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 57 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 47 kDa,65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

References

ab56301 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact ...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

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Thank you for your reply.
I have aligned the human angiopoietin 1 and 2 proteins and the overall sequence homology is only 61%.
I would recommend ab64015, since the immunogen does not align well with Ang1 (63% homology)
http://www.abcam....

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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