Anti-Angiopoietin 3 antibody [RM0073-2H34] (ab86047)

Overview

  • Product nameAnti-Angiopoietin 3 antibody [RM0073-2H34]
  • Description
    Rat monoclonal [RM0073-2H34] to Angiopoietin 3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Mouse recombinant Angiopoietin 3.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesThe IgG fraction of culture supernatant was purified by protein A/G affinity chromatography and filter sterilised through a 0.2 µm filter.
  • ClonalityMonoclonal
  • Clone numberRM0073-2H34
  • IsotypeIgG2
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 58 kDa.

Target

  • RelevanceAngiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The mechanism by which they contribute to angiogenesis is thought to involve regulation of endothelial cell interactions with supporting perivascular cells. Angiopoietin 3 (ANG3), also called ANG4, might represent counterparts in mouse and human. Based on chromosomal analysis, mouse ANG3 is thought to be an ortholog of human ANG4. Human ANG4 acts as an agonist that binds and activates Tie2, whereas mouse ANG3 is likely to be a Tie2 antagonist, exerting agonist or antagonist activities depending on the cell context. Mouse ANG3 is expressed in various mouse tissues. Mouse angiopoietin 3 is a secreted glycoprotein and member of the angiopoietin family. It has an N terminal coiled-coil domain and a Cbterminal fibrinogen like domain. The coiled coil domains mediate ligand homo oligomerization and the fibrinogen like domains mediate ligand activity. Recombinant mouse angiopoietin 3 has a calculated molecular mass of 58 kDa. Due to glycosylation, the recombinant protein (containing 3 peptides) has an apparent molecular mass of 85-90 kDa in SDS-PAGE under reducing conditions. Mouse angiopoietin 3 shares 47%, 46%, and 54% sequence identity with mouse ANG1, mouse ANG2, and human ANG4, respectively.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • AGP4 antibody
    • ANG 3 antibody
    • ANG 4 antibody
    • ANG3 antibody
    • ANG4 antibody
    • Angiopoietin 4 antibody
    • ANGPT 4 antibody
    • ANGPT4 antibody
    • MGC138181 antibody
    • MGC138183 antibody
    see all

References for Anti-Angiopoietin 3 antibody [RM0073-2H34] (ab86047)

ab86047 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"