Overview

  • Product name
    Anti-Angiopoietin-like 4 antibody
    See all Angiopoietin-like 4 primary antibodies
  • Description
    Rabbit polyclonal to Angiopoietin-like 4
  • Specificity
    Detects fasting-induced adipose factor (FIAF) from rat adipose tissue.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Mouse Angiopoietin-like 4 aa 80-94 (N terminal).
    Sequence:

    CQGPKGKDAPFKDSE


    (Peptide available as ab4936)

  • Positive control
    • Rat adipose tissue extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab2920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Can be blocked with Angiopoietin-like 4 peptide (ab4936).

By Western blot, this antibody detects an ~50 kDa protein representing FIAF from rat adipose tissue extracts.

Target

  • Function
    Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorgenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation.
  • Tissue specificity
    Expressed at high levels in the placenta, heart, liver, muscle, pancreas and lung but expressed poorly in the brain and kidney.
  • Sequence similarities
    Contains 1 fibrinogen C-terminal domain.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localization
    Secreted. Secreted > extracellular space > extracellular matrix. The unprocessed form interacts with the extracellular matrix. This may constitute a dynamic reservoir, a regulatory mechanism of the bioavailability of ANGPTL4.
  • Information by UniProt
  • Database links
  • Alternative names
    • Angiopoietin like 4 antibody
    • Angiopoietin related protein 4 antibody
    • Angiopoietin-like protein 4 antibody
    • Angiopoietin-related protein 4 antibody
    • ANGL4_HUMAN antibody
    • ANGPT L2 antibody
    • ANGPT L4 antibody
    • ANGPTL2 antibody
    • Angptl4 antibody
    • ARP4 antibody
    • Fasting induced adipose factor antibody
    • FIAF antibody
    • HARP antibody
    • Hepatic angiopoietin related protein antibody
    • Hepatic fibrinogen/angiopoietin related protein antibody
    • Hepatic fibrinogen/angiopoietin-related protein antibody
    • HFARP antibody
    • NL2 antibody
    • Peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin related protein antibody
    • PGAR antibody
    • pp1158 antibody
    • PPARG angiopoietin related protein antibody
    • PSEC0166 antibody
    • TGQTL antibody
    • UNQ171 antibody
    • Weakly similar to angiopoietin 1 [H.sapiens] antibody
    see all

Images

  • Western blot on FIAF in rat adipose tissue extract using ab2920.

References

This product has been referenced in:
  • Rodrigues T  et al. Methylglyoxal-induced glycation changes adipose tissue vascular architecture, flow and expansion, leading to insulin resistance. Sci Rep 7:1698 (2017). WB ; Rat . Read more (PubMed: 28490763) »
  • Villa L  et al. Effects and mechanisms of angiotensin II receptor blockade with telmisartan in a normotensive model of mesangioproliferative nephritis. Nephrol Dial Transplant : (2011). Read more (PubMed: 21414972) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Non-reduced Denaturing (12.5%)
Sample
Mouse Tissue lysate - other (white fat tissue)
Specification
white fat tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jan 22 2014

Thank you fro bringing this to our attention. Are you seeing a band at 50 kDa, and if so, is it more intense than the other bands? Where do the other bands appear in the blot? If you can attach an image, the may help me understand what is going on. Read More

Thank you for your inquiry. We do not assign strict expiration dates for our products, however we follow general guidelines: Antibodies stored at 4C may be viable up to 1 year, those stored at -20C may be viable for up to 5 years, and those stored at -...

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Thank you for your enquiry. The predicted KDa that this target should run at (Swiss Prot) is 46KDa, there is no indication of an alternate isoform of this target that I have been able to source. The paper cited below appears to indicate that the full l...

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Thank you for your enquiry and patience. To our knowledge, this antibody has only been tested for application in Western blotting, and so we don't have any information about how ab2920 works in IP. Have you done a positive control? I would suggest runn...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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