Angiotensin Converting Enzyme 1 antibody - Aminoterminal end catalytic domain (ab28311)

Product Name 

Angiotensin Converting Enzyme 1 antibody - Aminoterminal end catalytic domain

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Angiotensin Converting Enzyme 1 - Aminoterminal end catalytic domain

Immunogen 

The antibody is made to a synthetic peptide based on the first peptidase unit of human ACE1 (Human).

(Peptide available as ab41278.)

Reacts with 
(species key)

Hu

Specificity 

ab28311 recognizes metallopeptidase ACE1.

We have a range of domain specific antibodies for this target. For a full list please see all Angiotensin Converting Enzyme 1 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 150 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

ACE1 is found in the lung, kidney, heart, pancreas, prostate, testis, small intestine and colon, and is found in cell lysates and as shed or processed forms in cell culture media and in biological fluids.

Cellular localization 

Type I membrane protein

Research areas 

Cardiovascular >> Vasculature >> Vasoconstriction >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ACEs
Signal Transduction >> Metabolism >> Vitamins / Minerals
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Cardiovascular >> Blood >> Serum Proteins

Relevance 

ACE converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilatator. ACE exists in two forms, a 170KD somatic form and a 90KD germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Sucrose, PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

The antibody has been peptide-affinity purified and concentrated.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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