Anti-Angiotensin Converting Enzyme 1 antibody - Carboxyterminal end (ab39172)

Overview

  • Product nameAnti-Angiotensin Converting Enzyme 1 antibody - Carboxyterminal end
    See all Angiotensin Converting Enzyme 1 primary antibodies
  • Description
    Rabbit polyclonal to Angiotensin Converting Enzyme 1 - Carboxyterminal end
  • SpecificityThis antibody is specific for Angiotensin Converting Enzyme 1.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide based on the carboxy end of the second peptidase unit of human Angiotensin Converting Enzyme 1.

    (Peptide available as ab41280.)

Properties

Applications

Our Abpromise guarantee covers the use of ab39172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Detects a band of approximately 175-184kDa, due to glycosylation and other post-translational modifications (predicted molecular weight: 150 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionConverts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.
    • Tissue specificityUbiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis.
    • Involvement in diseaseIschemic stroke (ISCHSTR) [MIM:601367]: A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
      Renal tubular dysgenesis (RTD) [MIM:267430]: Autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Note=The disease is caused by mutations affecting the gene represented in this entry.
      Microvascular complications of diabetes 3 (MVCD3) [MIM:612624]: Pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
      Intracerebral hemorrhage (ICH) [MIM:614519]: A pathological condition characterized by bleeding into one or both cerebral hemispheres including the basal ganglia and the cerebral cortex. It is often associated with hypertension and craniocerebral trauma. Intracerebral bleeding is a common cause of stroke. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
    • Sequence similaritiesBelongs to the peptidase M2 family.
    • Post-translational
      modifications
      Phosphorylated by CK2 on Ser-1299; which allows membrane retention.
    • Cellular localizationSecreted and Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ACE 1 antibody
      • ACE antibody
      • ACE T antibody
      • ACE_HUMAN antibody
      • ACE1 antibody
      • Angiotensin converting enzyme somatic isoform antibody
      • Angiotensin converting enzyme testis specific isoform antibody
      • Angiotensin I converting enzyme 1 antibody
      • Angiotensin I converting enzyme antibody
      • Angiotensin I converting enzyme peptidyl dipeptidase A 1 antibody
      • angiotensin I converting enzyme peptidyl-dipeptidase A 1 transcript antibody
      • Angiotensin-converting enzyme antibody
      • Carboxycathepsin antibody
      • CD 143 antibody
      • CD143 antibody
      • CD143 antigen antibody
      • DCP 1 antibody
      • DCP antibody
      • DCP1 antibody
      • Dipeptidyl carboxypeptidase 1 antibody
      • Dipeptidyl carboxypeptidase I antibody
      • Kininase II antibody
      • MGC26566 antibody
      • MVCD3 antibody
      • Peptidase P antibody
      • Peptidyl dipeptidase A antibody
      • soluble form antibody
      • Testicular ECA antibody
      see all

    Anti-Angiotensin Converting Enzyme 1 antibody - Carboxyterminal end images

    • All lanes : Anti-Angiotensin Converting Enzyme 1 antibody - Carboxyterminal end (ab39172) at 1/1000 dilution

      Lane 1 : ACE1 at 0.05 µg
      Lane 2 : ACE1 at 0.01 µg
      Lane 3 : ACE1 at 0.001 µg


      Predicted band size : 150 kDa
      Observed band size : 170 kDa (why is the actual band size different from the predicted?)

    References for Anti-Angiotensin Converting Enzyme 1 antibody - Carboxyterminal end (ab39172)

    This product has been referenced in:
    • Dahan D  et al. Induction of angiotensin-converting enzyme after miR-143/145 deletion is critical for impaired smooth muscle contractility. Am J Physiol Cell Physiol 307:C1093-101 (2014). Read more (PubMed: 25273883) »
    • Goyal R  et al. Antenatal maternal protein deprivation: sexually dimorphic programming of the pancreatic renin-angiotensin system. J Renin Angiotensin Aldosterone Syst 14:137-45 (2013). Read more (PubMed: 22898440) »

    See all 3 Publications for this product

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