Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762)

Overview

  • Product nameAnti-Angiotensin Converting Enzyme 1 antibody [EPR2757]
    See all Angiotensin Converting Enzyme 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR2757] to Angiotensin Converting Enzyme 1
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    within Human Angiotensin Converting Enzyme 1 aa 1250 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P12821

  • Positive control
    • Fetal kidney, fetal heart and fetal lung lysates; human kidney and spleen tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Applications

Our Abpromise guarantee covers the use of ab75762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 195 kDa (predicted molecular weight: 150 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notesIs unsuitable for ICC or IP.
  • Target

    • FunctionConverts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.
    • Tissue specificityUbiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis.
    • Involvement in diseaseIschemic stroke (ISCHSTR) [MIM:601367]: A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
      Renal tubular dysgenesis (RTD) [MIM:267430]: Autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Note=The disease is caused by mutations affecting the gene represented in this entry.
      Microvascular complications of diabetes 3 (MVCD3) [MIM:612624]: Pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
      Intracerebral hemorrhage (ICH) [MIM:614519]: A pathological condition characterized by bleeding into one or both cerebral hemispheres including the basal ganglia and the cerebral cortex. It is often associated with hypertension and craniocerebral trauma. Intracerebral bleeding is a common cause of stroke. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
    • Sequence similaritiesBelongs to the peptidase M2 family.
    • Post-translational
      modifications
      Phosphorylated by CK2 on Ser-1299; which allows membrane retention.
    • Cellular localizationSecreted and Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ACE 1 antibody
      • ACE antibody
      • ACE T antibody
      • ACE_HUMAN antibody
      • ACE1 antibody
      • Angiotensin converting enzyme somatic isoform antibody
      • Angiotensin converting enzyme testis specific isoform antibody
      • Angiotensin I converting enzyme 1 antibody
      • Angiotensin I converting enzyme antibody
      • Angiotensin I converting enzyme peptidyl dipeptidase A 1 antibody
      • angiotensin I converting enzyme peptidyl-dipeptidase A 1 transcript antibody
      • Angiotensin-converting enzyme antibody
      • Carboxycathepsin antibody
      • CD 143 antibody
      • CD143 antibody
      • CD143 antigen antibody
      • DCP 1 antibody
      • DCP antibody
      • DCP1 antibody
      • Dipeptidyl carboxypeptidase 1 antibody
      • Dipeptidyl carboxypeptidase I antibody
      • Kininase II antibody
      • MGC26566 antibody
      • MVCD3 antibody
      • Peptidase P antibody
      • Peptidyl dipeptidase A antibody
      • soluble form antibody
      • Testicular ECA antibody
      see all

    Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] images

    • All lanes : Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762) at 1/1000 dilution

      Lane 1 : fetal kidney lysate
      Lane 2 : fetal heart lysate
      Lane 3 : fetal lung lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 150 kDa
      Observed band size : 195 kDa (why is the actual band size different from the predicted?)
    • ab75762 at 1/100 dilution staining Angiotensin Converting Enzyme 1 in human kidney by Immunohistochemistry, Paraffin-embedded tissue.
    • ab75762 at 1/100 dilution staining Angiotensin Converting Enzyme 1 in human spleen by Immunohistochemistry, Paraffin-embedded tissue. Note positive staining of endothelial cells.

    References for Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762)

    This product has been referenced in:
    • Yang J  et al. Pathological Ace2-to-Ace enzyme switch in the stressed heart is transcriptionally controlled by the endothelial Brg1-FoxM1 complex. Proc Natl Acad Sci U S A 113:E5628-35 (2016). Read more (PubMed: 27601681) »
    • Zhou L  et al. Multiple Genes of the Renin-Angiotensin System Are Novel Targets of Wnt/ß-Catenin Signaling. J Am Soc Nephrol N/A:N/A (2014). Read more (PubMed: 25012166) »

    See all 3 Publications for this product

    Product Wall

    Wie besprochen habe ich eine kostenlose Ersatzlieferung für Sie in Auftrag gegeben. Sie hat die Referenznummer 1155271 und sollte morgen bei Ihnen ankommen.

    Das Molekulargewicht kann man folgendermaßen errechnen: Öffnen Sie die SwissProt Lin...

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    1. Ich denke, Sie detektieren das richtige Protei...

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    Vielen Dank für Ihren Anruf.

    Es tut mir leid zu hören, dass Sie Probleme mit diesen Antikörpern haben.

    Ich habe unseren Fragebogen als Word-Dokument an diese E-Mail angehängt. Durch das Ausfüllen des Frage...

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    Thank you for contacting us. The immunogen of ab75762 shares 57% identity with the testis-specific isoform of Angiotensin Converting Enzyme 1. Thus, I would not predict that it would recognize this isoform. I hope this information is helpful to ...

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