Anti-Angiotensin Converting Enzyme 2 antibody (ab15348)

Overview

  • Product nameAnti-Angiotensin Converting Enzyme 2 antibody
    See all Angiotensin Converting Enzyme 2 primary antibodies
  • Description
    Rabbit polyclonal to Angiotensin Converting Enzyme 2
  • Tested applicationsSuitable for: IHC-Fr, ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Cat, Human, Ferret, Macaque Monkey
  • Immunogen

    Synthetic peptide:

    CKGENNPGFQNTDDVQTSF

    , corresponding to C terminal amino acids 788-805 of Human ACE2. (Peptide available as ab15352.)

  • Positive control
    • Human kidney or human testis cell lysate
  • General notes


    No cross reactivity to ACE1.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab15348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 97 kDa).Can be blocked with Human Angiotensin Converting Enzyme 2 peptide (ab15352).
IHC-P Use at an assay dependent concentration.

Target

  • FunctionCarboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.
  • Tissue specificityExpressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system.
  • Sequence similaritiesBelongs to the peptidase M2 family.
  • Post-translational
    modifications
    N-glycosylation on Asn-90 may limit SARS infectivity.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACE 2 antibody
    • ACE related carboxypeptidase antibody
    • ACE-related carboxypeptidase antibody
    • ACE2 antibody
    • ACE2_HUMAN antibody
    • ACEH antibody
    • Angiotensin converting enzyme 2 antibody
    • Angiotensin converting enzyme homolog antibody
    • Angiotensin converting enzyme like protein antibody
    • Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2 antibody
    • Angiotensin I converting enzyme 2 antibody
    • Angiotensin-converting enzyme homolog antibody
    • DKFZP434A014 antibody
    • EC 3.4.17 antibody
    • metalloprotease MPROT 15 antibody
    • Metalloprotease MPROT15 antibody
    • OTTHUMP00000022963 antibody
    • Processed angiotensin-converting enzyme 2 antibody
    see all

Anti-Angiotensin Converting Enzyme 2 antibody images



  • Predicted band size : 97 kDa

    Western blot analysis of ACE2 in human kidney lysate with ab15438 at 0.5  μg/mL (lane A), 1  μg/mL (lane B), and 2  μg/mL (lane C).

  • ab15348 at 2µg/ml staining Angiotensin Converting Enzyme 2 in human kidney by IHC

  • ICC/IF image of ab15348 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15348, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab15348 staining angiotensin converting enzyme 2 in human thoracic aortic sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone, permeabilized and blocked with a proprietary kit. Samples were incubated with primary antibody (2 µg/ml in TBST + Sodium azide) for 1 hour at 25°C. An undiluted HRP-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.

    See Abreview

References for Anti-Angiotensin Converting Enzyme 2 antibody (ab15348)

This product has been referenced in:
  • Thatcher SE  et al. Angiotensin-Converting Enzyme 2 Decreases Formation and Severity of Angiotensin II-Induced Abdominal Aortic Aneurysms. Arterioscler Thromb Vasc Biol 34:2617-23 (2014). Read more (PubMed: 25301841) »
  • Honorato-Sampaio K  et al. Evidence that angiotensin-(1-7) is an intermediate of gonadotrophin-induced oocyte maturation in the rat preovulatory follicle. Exp Physiol 97:642-50 (2012). IHC-P ; Rat . Read more (PubMed: 22247282) »

See all 6 Publications for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Non-Denaturing (Native) (Reduced Non-Denaturing (Native) (4-20% Tris-HCl pre-cast gels))
Sample Human Tissue lysate - whole (Brain)
Specification Brain
Treatment Diluted 1:2 in sample buffer treated with Þ-mercaptoethanol
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Miss. Laura Palmer

Verified customer

Submitted Sep 06 2013

Hello and thank you for contacting Abcam. Although these products should be suitable to work together with ab15352 blocking ab15248, we do not have the WB image on file. Please let me know if I can be of further assistance.

Thank you for contacting us. We do not offer conjugation services, unfortunately, but rather offer the conjugation kits (www.abcam.com/EasyLink). However, we do not carry Alexa Fluor conjugation kits or Alexa Fluor conjugated secondary antibodies. ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (human thoraci aortic section- fresh frozen)
Specification human thoraci aortic section- fresh frozen
Fixative Acetone
Permeabilization Yes - NOVACASTRA KIT COMPONENT
Blocking step (agent) for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 16 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Ferret Tissue sections (respiratory tract)
Specification respiratory tract
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citric acid buffer pH 6.0
Permeabilization No
Username

Abcam user community

Verified customer

Submitted Sep 08 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Cat Tissue sections (respiratory tract)
Specification respiratory tract
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citric acid buffer pH 6.0
Permeabilization No
Username

Abcam user community

Verified customer

Submitted Sep 08 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Brain)
Specification Brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 1%
Username

Miss. Laura Palmer

Verified customer

Submitted May 22 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"