Anti-Angiotensin Converting Enzyme 2 antibody (ab15348)

Overview

  • Product name
    Anti-Angiotensin Converting Enzyme 2 antibody
    See all Angiotensin Converting Enzyme 2 primary antibodies
  • Description
    Rabbit polyclonal to Angiotensin Converting Enzyme 2
  • Tested applications
    Suitable for: IHC-Fr, ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Cat, Human, Ferret, Macaque monkey
  • Immunogen

    Synthetic peptide:

    CKGENNPGFQNTDDVQTSF

    , corresponding to C terminal amino acids 788-805 of Human ACE2. (Peptide available as ab15352.)

  • Positive control
    • Human kidney or human testis cell lysate
  • General notes


    No cross reactivity to ACE1.

Properties

Applications

Our Abpromise guarantee covers the use of ab15348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 97 kDa).Can be blocked with Human Angiotensin Converting Enzyme 2 peptide (ab15352).
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.
  • Tissue specificity
    Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system.
  • Sequence similarities
    Belongs to the peptidase M2 family.
  • Post-translational
    modifications
    N-glycosylation on Asn-90 may limit SARS infectivity.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACE 2 antibody
    • ACE related carboxypeptidase antibody
    • ACE-related carboxypeptidase antibody
    • ACE2 antibody
    • ACE2_HUMAN antibody
    • ACEH antibody
    • Angiotensin converting enzyme 2 antibody
    • Angiotensin converting enzyme homolog antibody
    • Angiotensin converting enzyme like protein antibody
    • Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2 antibody
    • Angiotensin I converting enzyme 2 antibody
    • Angiotensin-converting enzyme homolog antibody
    • DKFZP434A014 antibody
    • EC 3.4.17 antibody
    • metalloprotease MPROT 15 antibody
    • Metalloprotease MPROT15 antibody
    • OTTHUMP00000022963 antibody
    • Processed angiotensin-converting enzyme 2 antibody
    see all

Anti-Angiotensin Converting Enzyme 2 antibody images



  • Predicted band size : 97 kDa

    Western blot analysis of ACE2 in human kidney lysate with ab15438 at 0.5  μg/mL (lane A), 1  μg/mL (lane B), and 2  μg/mL (lane C).

  • ab15348 at 2µg/ml staining Angiotensin Converting Enzyme 2 in human kidney by IHC

  • ICC/IF image of ab15348 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15348, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab15348 staining angiotensin converting enzyme 2 in human thoracic aortic sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone, permeabilized and blocked with a proprietary kit. Samples were incubated with primary antibody (2 µg/ml in TBST + Sodium azide) for 1 hour at 25°C. An undiluted HRP-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.

    See Abreview

References for Anti-Angiotensin Converting Enzyme 2 antibody (ab15348)

This product has been referenced in:
  • Wang G  et al. Enalapril protects against myocardial ischemia/reperfusion injury in a swine model of cardiac arrest and resuscitation. Int J Mol Med 38:1463-1473 (2016). WB, IHC ; Pig . Read more (PubMed: 27633002) »
  • Kehoe PG  et al. Angiotensin-converting enzyme 2 is reduced in Alzheimer's disease in association with increasing amyloid-ß and tau pathology. Alzheimers Res Ther 8:50 (2016). IHC-P ; Human . Read more (PubMed: 27884212) »

See all 8 Publications for this product

Product Wall

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Non-Denaturing (Native) (Reduced Non-Denaturing (Native) (4-20% Tris-HCl pre-cast gels))
Sample
Human Tissue lysate - whole (Brain)
Specification
Brain
Treatment
Diluted 1:2 in sample buffer treated with Þ-mercaptoethanol
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Miss. Laura Palmer

Verified customer

Submitted Sep 06 2013

Hello and thank you for contacting Abcam. Although these products should be suitable to work together with ab15352 blocking ab15248, we do not have the WB image on file. Please let me know if I can be of further assistance.

Thank you for contacting us. We do not offer conjugation services, unfortunately, but rather offer the conjugation kits (www.abcam.com/EasyLink). However, we do not carry Alexa Fluor conjugation kits or Alexa Fluor conjugated secondary antibodies. ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (human thoraci aortic section- fresh frozen)
Specification
human thoraci aortic section- fresh frozen
Fixative
Acetone
Permeabilization
Yes - NOVACASTRA KIT COMPONENT
Blocking step
(agent) for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 16 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Ferret Tissue sections (respiratory tract)
Specification
respiratory tract
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric acid buffer pH 6.0
Permeabilization
No
Username

Abcam user community

Verified customer

Submitted Sep 08 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cat Tissue sections (respiratory tract)
Specification
respiratory tract
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric acid buffer pH 6.0
Permeabilization
No
Username

Abcam user community

Verified customer

Submitted Sep 08 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain)
Specification
Brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 1%
Username

Miss. Laura Palmer

Verified customer

Submitted May 22 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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