Angiotensin Converting Enzyme 2 antibody - Carboxyterminal end catalytic domain (ab38941)

Product Name 

Angiotensin Converting Enzyme 2 antibody - Carboxyterminal end catalytic domain

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Angiotensin Converting Enzyme 2 - Carboxyterminal end catalytic domain

Immunogen 

Synthetic peptide corresponding to the carboxy end of the catalytic domain of Human Angiotensin Converting Enzyme 2, between the zinc binding site and the sheddase cleavage site.

(Peptide available as ab41283.)

Reacts with 

Hu

Predicted to react 
(species key)

Ms
(Due to sequence homology)

Specificity 

ab38941 recognises the Carboxy end of Catalytic domain of Angiotensin Converting Enzyme 2.

We have a range of domain specific antibodies for this target. For a full list please see all Angiotensin Converting Enzyme 2 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. The full-length sequence for ACE-2 encodes an 805 amino acid protein with predicted mass of 92kDa. However,glycosylation and other post-translational modifications make the apparent molecular weight on SDS PAGE gels larger, about 120 kDa by Western blot. Many smaller forms may be seen on Western blot of culture media and biological fluid. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cell membrane; single pass type I membrane protein and secreted (processed form).

Research areas 

Cardiovascular >> Vasculature >> Vasoconstriction >> Other
Cardiovascular >> Heart >> Contractility >> Inotropics
Cardiovascular >> Blood >> Blood Pressure regulation
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ACEs

Relevance 

Angiotensin converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology. In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently ACE2 has also been shown to be a functional receptor of the SARS coronavirus. The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1 7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function. ACE1 inhibitors such as catopril and lisinopril do not block ACE2 activity. ACE2 is restricted to the testis, heart, kidney and colon. The full length sequence for ACE2 encodes an 805 amino acid protein with predicted mass of 92.46 kDa and a pI of 5.22.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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