Anti-Angiotensin Converting Enzyme 2 antibody [EPR4435(2)] (ab108252)


  • Product name
    Anti-Angiotensin Converting Enzyme 2 antibody [EPR4435(2)]
    See all Angiotensin Converting Enzyme 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR4435(2)] to Angiotensin Converting Enzyme 2
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in the extracellular domain of Human Angiotensin Converting Enzyme 2.

  • Positive control
    • Fetal kidney and Human testis lysate; Human kidney tissue; Human testis tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents



Our Abpromise guarantee covers the use of ab108252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 92 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.
    • Tissue specificity
      Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system.
    • Sequence similarities
      Belongs to the peptidase M2 family.
    • Post-translational
      N-glycosylation on Asn-90 may limit SARS infectivity.
    • Cellular localization
      Secreted and Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ACE 2 antibody
      • ACE related carboxypeptidase antibody
      • ACE-related carboxypeptidase antibody
      • ACE2 antibody
      • ACE2_HUMAN antibody
      • ACEH antibody
      • Angiotensin converting enzyme 2 antibody
      • Angiotensin converting enzyme homolog antibody
      • Angiotensin converting enzyme like protein antibody
      • Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2 antibody
      • Angiotensin I converting enzyme 2 antibody
      • Angiotensin-converting enzyme homolog antibody
      • DKFZP434A014 antibody
      • EC 3.4.17 antibody
      • metalloprotease MPROT 15 antibody
      • Metalloprotease MPROT15 antibody
      • OTTHUMP00000022963 antibody
      • Processed angiotensin-converting enzyme 2 antibody
      see all


    • All lanes : Anti-Angiotensin Converting Enzyme 2 antibody [EPR4435(2)] (ab108252) at 1/1000 dilution

      Lane 1 : Fetal kidney lysate
      Lane 2 : Human testis lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 92 kDa
    • ab108252, at 1/100 dilution, staining Angiotensin Converting Enzyme 2 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
    • ab108252, at 1/100 dilution, staining Angiotensin Converting Enzyme 2 in paraffin-embedded Human testis tissue by Immunohistochemistry


    This product has been referenced in:
    • Liu Q  et al. miRNA-200c-3p is crucial in acute respiratory distress syndrome. Cell Discov 3:17021 (2017). Read more (PubMed: 28690868) »
    • Shen L  et al. Effects of 1,25-dihydroxyvitamin D3 on the local bone renin-angiotensin system in a murine model of glucocorticoid-induced osteoporosis. Exp Ther Med 13:3297-3304 (2017). Read more (PubMed: 28587403) »

    See all 4 Publications for this product

    Customer reviews and Q&As

    Yes of course, thanks for your reply.

    We don't have any specific recommendations for western blotting protocol for ab32212. Although a good starting dilution for western blotting using ab32212 is noted as 1:5000.

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    I'm sorry but I don't quite understand your question. What is it that you requested for ab32213 and ab52174?

    Thanks for the additional details.

    Thank you for your enquiry.

    i have checked and none ofthese 3 antibodies have a specific western blot protocol associated with them, although typically a 1:1000 dilution, or 1ug/ml primaryantibody concentration works well as a starting point ...

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    Thank you for contacting us.

    The link to our list of buffer solutions and their composition is:

    The link to our WB protocol is:

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    Thanks for your email.

    For ab11734 I'd recommend starting with a dilution of 1: 250 and dilute ab87436 to 1:1000. The primary antibody diluent could be TBS with 0.05% Tween 20.

    I hope this is helpful. Please contact me again if you ...

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    Thank you for your enquiry.

    Here is an antibody that has be used in western blotting and IHC on rat samples for ACE 1:

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