Anti-Angiotensin I antibody (ab8051)

Overview

  • Product nameAnti-Angiotensin I antibody
    See all Angiotensin I primary antibodies
  • Description
    Rabbit polyclonal to Angiotensin I
  • SpecificitySpecificity of the antisera is confirmed by its ability to bind and precipitate labeled angiotensin I and to be adsorbed by angiotensin. Native angiotensin competes with such binding, while human whole plasma or plasma proteins do not. The antiserum also reacts with angiotensin II (Reactivity in competitive ELISA was shown : 80% to Angiotensin II; 100% to Angiotensin I)
  • Tested applicationsSuitable for: ELISAmore details
    Unsuitable for: IHC
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide:

    DRVYIHPFHL

    conjugated to BSA by a CDI linker, corresponding to N terminal amino acids 34-43 of Human Angiotensin I.

  • General notesThe enzyme renin cleaves angiotensinogen to produce an inactive prohormone, angiotensin I; the latter is transformed to the active molecule angiotensin II by another enzyme, angiotensin-converting enzyme

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab8051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
  • Application notesELISA: Use at an assay dependent dilution.

    Is unsuitable for IHC.

    Not yet tested in other applications.
    A starting dilution of 1/100 is recommended. Please note that optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionEssential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. In response to lowered blood pressure, the enzyme renin cleaves angiotensinogen to produce angiotensin-1 (angiotensin 1-10). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2 (angiotensin 1-8). Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3 (angiotensin 2-8), angiotensin-4 (angiotensin 3-8). Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin). Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2.
      Angiotensin-2 acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone.
      Angiotensin-3 stimulates aldosterone release.
      Angiotensin 1-7 is a ligand for the G-protein coupled receptor MAS1 (By similarity). Has vasodilator and antidiuretic effects (By similarity). Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets.
    • Tissue specificityExpressed by the liver and secreted in plasma.
    • Involvement in diseaseGenetic variations in AGT are a cause of susceptibility to essential hypertension (EHT) [MIM:145500]. Essential hypertension is a condition in which blood pressure is consistently higher than normal with no identifiable cause.
      Defects in AGT are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
    • Sequence similaritiesBelongs to the serpin family.
    • Post-translational
      modifications
      Beta-decarboxylation of Asp-34 in angiotensin-2, by mononuclear leukocytes produces alanine. The resulting peptide form, angiotensin-A, has the same affinity for the AT1 receptor as angiotensin-2, but a higher affinity for the AT2 receptor.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • AGT antibody
      • Alpha 1 antiproteinase antitrypsin antibody
      • Ang I antibody
      • Ang II antibody
      • Ang III antibody
      • Angiotensin 1 antibody
      • Angiotensin I antibody
      • Angiotensin II antibody
      • Angiotensin III antibody
      • Angiotensin-3 antibody
      • Angiotensinogen antibody
      • Angiotensinogen serpin peptidase inhibitor clade A member 8 antibody
      • ANGT_HUMAN antibody
      • ANHU antibody
      • Des-Asp[1]-angiotensin II antibody
      • FLJ92595 antibody
      • FLJ97926 antibody
      • OTTHUMP00000035878 antibody
      • Pre angiotensinogen antibody
      • Serine or cysteine proteinase inhibitor antibody
      • Serpin A8 antibody
      • SERPINA8 antibody
      see all

    References for Anti-Angiotensin I antibody (ab8051)

    ab8051 has not yet been referenced specifically in any publications.

    Product Wall

    Reactivity in competitive ELISA was shown: 80% to Angiotensin II 100% to Angiotensin I

    This product is provided as whole antiserum, and not as a purified immunoglobulin. Thus, there are other proteins present in the sample, especially albumin. The antibody has been tested in ELISA and we guarantee its use in this application. Have y...

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    First I would like to confirm that AB8051 has been tested and characterized for Immunoassay so it should work for RIA. The recommended compatible secondary antibodies are listed on the datasheet of this antibody, please have a look at it. Ab7019 is...

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    The protein and Ig concentrations of this antibody have not been assessed.

    Specificity of the antiserum is confirmed by its ability to bind labelled Angiotensin I in precipitation, partition, or adsorption assays. Native Angiotensin I competes with such binding, while human whole plasma or plasma proteins do not. The antiseru...

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    As far as we are aware, this antibody has not been tested on angiotensinogen.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"