Anti-Angiotensin I antibody [BGN/KA/4H] (ab54212)

Overview

  • Product nameAnti-Angiotensin I antibody [BGN/KA/4H]
    See all Angiotensin I primary antibodies
  • Description
    Mouse monoclonal [BGN/KA/4H] to Angiotensin I
  • SpecificityThis antibody does not react with angiotensin II, III or angiotensinogen in ELISA.
  • Tested applicationsSuitable for: ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Marmoset (common), Orangutan
  • Immunogen

    Synthetic peptide:

    PFHLVIHNES(-C)

    , corresponding to amino acids 40-49 of Human Angiotensinogen precursor conjugated to HMW carrier protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab54212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution. In a simple indirect ELISA, using 1.0 ug of Angiotensin I coated per well. 50% binding was observed at an antibody dilution of 1/12K. Under the same conditions, the end point titre was observed at an antibody dilution of 1/100K.

Target

  • FunctionEssential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. In response to lowered blood pressure, the enzyme renin cleaves angiotensinogen to produce angiotensin-1 (angiotensin 1-10). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2 (angiotensin 1-8). Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3 (angiotensin 2-8), angiotensin-4 (angiotensin 3-8). Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin). Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2.
    Angiotensin-2 acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone.
    Angiotensin-3 stimulates aldosterone release.
    Angiotensin 1-7 is a ligand for the G-protein coupled receptor MAS1 (By similarity). Has vasodilator and antidiuretic effects (By similarity). Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets.
  • Tissue specificityExpressed by the liver and secreted in plasma.
  • Involvement in diseaseGenetic variations in AGT are a cause of susceptibility to essential hypertension (EHT) [MIM:145500]. Essential hypertension is a condition in which blood pressure is consistently higher than normal with no identifiable cause.
    Defects in AGT are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
  • Sequence similaritiesBelongs to the serpin family.
  • Post-translational
    modifications
    Beta-decarboxylation of Asp-34 in angiotensin-2, by mononuclear leukocytes produces alanine. The resulting peptide form, angiotensin-A, has the same affinity for the AT1 receptor as angiotensin-2, but a higher affinity for the AT2 receptor.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • AGT antibody
    • Alpha 1 antiproteinase antitrypsin antibody
    • Ang I antibody
    • Ang II antibody
    • Ang III antibody
    • Angiotensin 1 antibody
    • Angiotensin I antibody
    • Angiotensin II antibody
    • Angiotensin III antibody
    • Angiotensin-3 antibody
    • Angiotensinogen antibody
    • Angiotensinogen serpin peptidase inhibitor clade A member 8 antibody
    • ANGT_HUMAN antibody
    • ANHU antibody
    • Des-Asp[1]-angiotensin II antibody
    • FLJ92595 antibody
    • FLJ97926 antibody
    • OTTHUMP00000035878 antibody
    • Pre angiotensinogen antibody
    • Serine or cysteine proteinase inhibitor antibody
    • Serpin A8 antibody
    • SERPINA8 antibody
    see all

References for Anti-Angiotensin I antibody [BGN/KA/4H] (ab54212)

ab54212 has not yet been referenced specifically in any publications.

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