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Our Abpromise guarantee covers the use of ab9391 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.|
|ELISA||Use at an assay dependent concentration.|
|WB||Use a concentration of 5 µg/ml. Use under non reducing condition. Detects a band of approximately 40 kDa. Use under non reducing conditions to attempt to keep some structure of the receptor. It is best to use RIPA buffer(50 mM Tris pH 7.5, 150 mM NaCl, 1% NP-40, 0.5% Deoxycholic acid, 0.1% SDS, 2 mM EDTA, 10 mM NaF, 10 ug/ml leupeptin, 10 ug/ml pepstatin, 20 ug/ml aprotinin). Do not use milk to block. Detects bands of approximately 40,45 and 60 kDa depending on glycosylation of the receptor. In addition, found to work at dilution of 1/400.|
|ICC/IF||Use at an assay dependent concentration. Used at a dilution of 1/20 for 1 hr incubation (see Abreview for further information).|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 24759991|
This image is courtesy of an abreview submitted by Laura Palmer.
Lanes 2,3 and 4 contain human brain tissue homogenates from different individuals.
ab9391 at 1/20 dilution staining Rat aortic smooth muscle cells by immunocytochemistry. The cells were paraformaldehyde fixed and incubated with the antibody for 1 hour. Bound antibody was detected using a Rhodamine Red X-conjugated goat anti-mouse antibody.
This image is courtesy of an anonymous Abreview.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"