Anti-Angiotensin II Type 1 Receptor antibody [1E10-1A9] (ab9391)

Overview

  • Product nameAnti-Angiotensin II Type 1 Receptor antibody [1E10-1A9]
    See all Angiotensin II Type 1 Receptor primary antibodies
  • Description
    Mouse monoclonal [1E10-1A9] to Angiotensin II Type 1 Receptor
  • SpecificityIt reacts with both the recombinant form as well as that expressed on transfected CHO cells and rat smooth muscle cells. We have received feedback that this antibody may not be specific for the antigen (see PubMed 22843090 on the References page).
  • Tested applicationsSuitable for: IHC-P, ELISA, IP, WB, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat, Rabbit, Human
    Does not react with: Dog
  • Immunogen

    Fusion protein: LNPL FYGFL GKKFK RYFL QLLKYI PPK AKSHSNL ST KMSTLSYR P SDNVSSSTK KPAPCF, corresponding to C terminal amino acids 297-356 of Human Angiotensin II Type 1 Receptor

Properties

Applications

Our Abpromise guarantee covers the use of ab9391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use a concentration of 5 µg/ml. Use under non reducing condition. Detects a band of approximately 40 kDa. Use under non reducing conditions to attempt to keep some structure of the receptor. It is best to use RIPA buffer(50 mM Tris pH 7.5, 150 mM NaCl, 1% NP-40, 0.5% Deoxycholic acid, 0.1% SDS, 2 mM EDTA, 10 mM NaF, 10 ug/ml leupeptin, 10 ug/ml pepstatin, 20 ug/ml aprotinin). Do not use milk to block. Detects bands of approximately 40,45 and 60 kDa depending on glycosylation of the receptor. In addition, found to work at dilution of 1/400.
ICC/IF Use at an assay dependent concentration. Used at a dilution of 1/20 for 1 hr incubation (see Abreview for further information).
IHC-Fr Use at an assay dependent concentration. PubMed: 24759991

Target

  • FunctionReceptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
  • Tissue specificityLiver, lung, adrenal and adrenocortical adenomas.
  • Involvement in diseaseDefects in AGTR1 are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications
    C-terminal Ser or Thr residues may be phosphorylated.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AG2S antibody
    • Agtr 1 antibody
    • Agtr1 antibody
    • AGTR1_HUMAN antibody
    • Agtr1a antibody
    • AGTR1B antibody
    • Ang II antibody
    • Angiotensin II receptor type 1 antibody
    • Angiotensin II type-1 receptor antibody
    • Angiotensin receptor 1 antibody
    • Angiotensin receptor 1B antibody
    • AT 1B antibody
    • AT 1r antibody
    • AT1 antibody
    • At1a antibody
    • AT1AR antibody
    • AT1B antibody
    • AT1BR antibody
    • AT1R antibody
    • AT2R1 antibody
    • AT2R1A antibody
    • AT2R1B antibody
    • HAT1R antibody
    • Type 1 angiotensin II receptor antibody
    • Type 1B angiotensin II receptor antibody
    • Type-1 angiotensin II receptor antibody
    see all

Anti-Angiotensin II Type 1 Receptor antibody [1E10-1A9] images

  • ab9391 at 1/20 dilution staining Rat aortic smooth muscle cells by immunocytochemistry. The cells were paraformaldehyde fixed and incubated with the antibody for 1 hour. Bound antibody was detected using a Rhodamine Red X-conjugated goat anti-mouse antibody.

    This image is courtesy of an anonymous Abreview.

    See Abreview

  • ab9391 staining Angiotensin II Type 1 Receptor in human brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed for 10 minutes using EDTA/ microwave. Samples were then blocked with 1% serum for 20 minutes followed by incubation with the primary antibody at a 1/200 dilution for 18 hours. An HRP-conjugated horse secondary was used at a 1/50 dilution.

References for Anti-Angiotensin II Type 1 Receptor antibody [1E10-1A9] (ab9391)

This product has been referenced in:
  • Yang Y  et al. miR-155 functions downstream of angiotensin II receptor subtype 1 and calcineurin to regulate cardiac hypertrophy. Exp Ther Med 12:1556-1562 (2016). WB ; Rat . Read more (PubMed: 27588076) »
  • Bai X  et al. Antiangiogenic treatment diminishes renal injury and dysfunction via regulation of local AKT in early experimental diabetes. PLoS One 9:e96117 (2014). IHC-FoFr, IHC-Fr ; Rat . Read more (PubMed: 24759991) »

See all 18 Publications for this product

Product Wall

Thank you for your enquiry.
The immunogen used to produce this antibody is a fusion protein including a 60 amino acid region of Angiotensin II Type 1 Receptor. Antibodies produced using relatively large immunogens like this do not have blocking pe...

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Thank you for contacting us again.
Unfortunately, we haven't established this antibody ourself for ICC, but we got the information from one of our customers, which can be found on our website:
http://www.abcam.com/Angiotensin-II-Type-1-Recept...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

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Thank you for your message and for providing this further information.
I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments and would be pleased to arrang...

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Thank you for contacting us.
The DAKO dilution buffer can be tried. However as we do not know the components so we cannot comment on how well it will be for antibody.
We recommend heat mediated antigen retrieval via the microwave method befor...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Brain)
Loading amount 30 µg
Specification Brain
Treatment Diluted 1:2 in sample buffer treated with ß-mercaptoethanol
Gel Running Conditions Reduced Non-Denaturing (Native) (4-20% Tris-HCl pre-cast gels)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Miss. Laura Palmer

Verified customer

Submitted Nov 26 2012

Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results this time.
The details provided will enable us to investigate this case and will provide us with vital information...

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I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
I am attaching ...

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Thank you for taking the time to contact us. I am sorry to hear you have some concerns regarding the results from this antibody.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for IHC-P and in ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rabbit Tissue lysate - whole (heart)
Loading amount 20 µg
Specification heart
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Oct 01 2009

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"