Anti-Angiotensin II Type 1 Receptor antibody (ab124505)
Key features and details
- Rabbit polyclonal to Angiotensin II Type 1 Receptor
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse
- Isotype: IgG
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Overview
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Product name
Anti-Angiotensin II Type 1 Receptor antibody
See all Angiotensin II Type 1 Receptor primary antibodies -
Description
Rabbit polyclonal to Angiotensin II Type 1 Receptor -
Host species
Rabbit -
Specificity
ab124505 is specific for Angiotensin II Type 1 Receptor (AGTR1) and will not cross-react with AGTR2. -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
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Positive control
- Mouse kidney tissue lysate; Mouse kidney tissue, mouse kidney cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124505 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 20 µg/ml.
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WB | (1) |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 41 kDa.Can be blocked with Angiotensin II Type 1 Receptor peptide (ab176148).
|
IHC-P |
Use a concentration of 2.5 µg/ml.
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Notes |
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ICC/IF
Use a concentration of 20 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 41 kDa.Can be blocked with Angiotensin II Type 1 Receptor peptide (ab176148). |
IHC-P
Use a concentration of 2.5 µg/ml. |
Target
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Function
Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. -
Tissue specificity
Liver, lung, adrenal and adrenocortical adenomas. -
Involvement in disease
Defects in AGTR1 are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsC-terminal Ser or Thr residues may be phosphorylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 11607 Mouse
- Entrez Gene: 11608 Mouse
- SwissProt: P29754 Mouse
- SwissProt: P29755 Mouse
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Alternative names
- AG2S antibody
- Agtr 1 antibody
- Agtr1 antibody
see all
Images
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Lane 1 : Anti-Angiotensin II Type 1 Receptor antibody (ab124505) at 1 µg/ml
Lane 2 : Anti-Angiotensin II Type 1 Receptor antibody (ab124505) at 2 µg/ml
All lanes : Mouse kidney tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 41 kDa -
Immunofluorescence of AGTR1 in mouse kidney cells using ab124505 at 20 ug/ml.
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Immunohistochemical analysis of mouse kidney tissue section labelling Angiotensin II Type 1 Receptor with ab124505, at 2.5 µg/ml.
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ab124505, at 2.5 µg/ml, staining Angiotensin II Type 1 Receptor in paraffin-embedded Mouse kidney tissue by Immunohistochemistry.
Protocols
Datasheets and documents
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Datasheet download
References (26)
ab124505 has been referenced in 26 publications.
- Wang YN et al. Shenkang injection improves chronic kidney disease by inhibiting multiple renin-angiotensin system genes by blocking the Wnt/β-catenin signalling pathway. Front Pharmacol 13:964370 (2022). PubMed: 36059935
- Hosseini-Dastgerdi H et al. Renal Denervation Influences Angiotensin II Types 1 and 2 Receptors. Int J Nephrol 2022:8731357 (2022). PubMed: 36262553
- Liu J et al. Aptamer-assisted two-point immobilized agonist-bound angiotensin II type 1 receptor for a second-site modulator discovery. iScience 25:105361 (2022). PubMed: 36339252
- Zhang F et al. Puerarin exhibits antiinflammatory properties in gunpowder smog-induced acute lung injury in rats via regulation of the renin-angiotensin system and the NF?B signaling pathway. Exp Ther Med 22:809 (2021). PubMed: 34093765
- Mishra JS & Kumar S Activation of angiotensin type 2 receptor attenuates testosterone-induced hypertension and uterine vascular resistance in pregnant rats†. Biol Reprod 105:192-203 (2021). PubMed: 33739377