Anti-Angiotensin II Type 1 Receptor antibody (ab18801)
- Datasheet
- Specific References (21)
- Protocols
Overview
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Product nameAnti-Angiotensin II Type 1 Receptor antibody
See all Angiotensin II Type 1 Receptor primary antibodies -
DescriptionRabbit polyclonal to Angiotensin II Type 1 Receptor
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Host speciesRabbit
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SpecificityThis antibody recognizes isoforms AT-1A and AT-1B of the Angiotensin II type 1 receptor (AT-1r) in rat. We have received feedback that this antibody may not be specific for the antigen (see PubMed 22843099 on the References page).
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Tested applicationsSuitable for: WB, IHC-Fr, IHC-FoFr, IHC-P, Electron Microscopymore details
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Species reactivityReacts with: Mouse, Rat, Cow, Dog, Pig
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Immunogen
Synthetic peptide:
PSDNMSSSAKKPASC
conjugated to KLH, corresponding to Internal sequence amino acids 341-355 of Angiotensin II Type 1 Receptor AT-1A. Synthetic peptide: SSSAKKSASFFEVE conjugated to KLH, corresponding to Internal sequence amino acids 346-359 of Angiotensin II Type 1 Receptor AT-1B.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesAntisera was affinity purified by passage through two affinity columns, one cross-linked to AT-1A and the other with AT-1B, resulting in specificity to the sequence PSDNMSSSAKKPASCFEVE (341-359).
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunohistochemistry kits
Applications
Our Abpromise guarantee covers the use of ab18801 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 41 kDa). | |
| IHC-Fr | Use at an assay dependent concentration. | |
| IHC-FoFr | Use at an assay dependent concentration. PubMed: 19706421 | |
| IHC-P | 1/100. | |
| Electron Microscopy | 1/500. |
Target
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FunctionReceptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
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Tissue specificityLiver, lung, adrenal and adrenocortical adenomas.
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Involvement in diseaseDefects in AGTR1 are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
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Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
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Post-translational
modificationsC-terminal Ser or Thr residues may be phosphorylated. -
Cellular localizationCell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 281607 Cow
- Entrez Gene: 11607 Mouse
- Entrez Gene: 11608 Mouse
- Entrez Gene: 24180 Rat
- Entrez Gene: 81638 Rat
- SwissProt: P29754 Mouse
- SwissProt: P29755 Mouse
- SwissProt: P25095 Rat
see all -
Alternative names
- AG2S antibody
- Agtr 1 antibody
- Agtr1 antibody
see all
Images
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Western blot - Anti-Angiotensin II Type 1 Receptor antibody (ab18801)This image is courtesy of an Abreview submitted by Dr sudhish mishraAll lanes : Anti-Angiotensin II Type 1 Receptor antibody (ab18801) at 1/800 dilution
Lane 1 : Dog heart whole tissue lysate at 50 µg
Lane 2 : Dog heart whole tissue lysate at 25 µg
Lane 3 : Dog heart whole tissue lysate at 10 µg
Lane 4 : Dog heart whole tissue lysate at 5 µg
Lane 5 : Dog heart whole tissue lysate at 2 µg
Secondary
All lanes : HRP conjugated donkey polycloanl antibody
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 45 kDa (why is the actual band size different from the predicted?)
Additional bands at: 32 kDa (possible non-specific binding)
Exposure time: 2 minutes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin II Type 1 Receptor antibody (ab18801)This image is courtesy of an Abreview submitted by Ms Andrea Dannullisab18801 staining Angiotensin II Type 1 in porcine kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 30 minutes at 20°C; antigen retrieval was by heat mediation with a citrate buffer. Samples were incubated with primary antibody (1/100 in PBS + Triton X-100 + goat serum) for 24 hours at 4°C. An HRP secondary antibody was used. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin II Type 1 Receptor antibody (ab18801)This image is courtesy of an Abreview submitted by Andrea Dannullisab18801 staining Angiotensin II Type 1 Receptor in Mouse kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 30 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in PBS + Triton X100) for 24 hours at 4°C. An undiluted HRP-conjugated anti-rabbit polyclonal was used as the secondary antibody.
Protocols
References
This product has been referenced in:
- Francis M et al. Editor's Highlight: Role of Spleen-Derived Macrophages in Ozone-Induced Lung Inflammation and Injury. Toxicol Sci 155:182-195 (2017). Read more (PubMed: 27708193) »
- Wang G et al. Enalapril protects against myocardial ischemia/reperfusion injury in a swine model of cardiac arrest and resuscitation. Int J Mol Med 38:1463-1473 (2016). WB, IHC ; Pig . Read more (PubMed: 27633002) »
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