Anti-Angiotensin II Type 1 Receptor antibody (ab18801)

Overview

  • Product nameAnti-Angiotensin II Type 1 Receptor antibody
    See all Angiotensin II Type 1 Receptor primary antibodies
  • Description
    Rabbit polyclonal to Angiotensin II Type 1 Receptor
  • SpecificityThis antibody recognizes isoforms AT-1A and AT-1B of the Angiotensin II type 1 receptor (AT-1r) in rat. We have received feedback that this antibody may not be specific for the antigen (see PubMed 22843099 on the References page).
  • Tested applicationsSuitable for: WB, IHC-Fr, IHC-FoFr, IHC-P, Electron Microscopymore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    PSDNMSSSAKKPASC

    conjugated to KLH, corresponding to Internal sequence amino acids 341-355 of Angiotensin II Type 1 Receptor AT-1A. Synthetic peptide: SSSAKKSASFFEVE conjugated to KLH, corresponding to Internal sequence amino acids 346-359 of Angiotensin II Type 1 Receptor AT-1B.

Properties

Applications

Our Abpromise guarantee covers the use of ab18801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 41 kDa).
IHC-Fr Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 19706421
IHC-P 1/100.
Electron Microscopy 1/500.

Target

  • FunctionReceptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
  • Tissue specificityLiver, lung, adrenal and adrenocortical adenomas.
  • Involvement in diseaseDefects in AGTR1 are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications
    C-terminal Ser or Thr residues may be phosphorylated.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AG2S antibody
    • Agtr 1 antibody
    • Agtr1 antibody
    • AGTR1_HUMAN antibody
    • Agtr1a antibody
    • AGTR1B antibody
    • Ang II antibody
    • Angiotensin II receptor type 1 antibody
    • Angiotensin II type-1 receptor antibody
    • Angiotensin receptor 1 antibody
    • Angiotensin receptor 1B antibody
    • AT 1B antibody
    • AT 1r antibody
    • AT1 antibody
    • At1a antibody
    • AT1AR antibody
    • AT1B antibody
    • AT1BR antibody
    • AT1R antibody
    • AT2R1 antibody
    • AT2R1A antibody
    • AT2R1B antibody
    • HAT1R antibody
    • Type 1 angiotensin II receptor antibody
    • Type 1B angiotensin II receptor antibody
    • Type-1 angiotensin II receptor antibody
    see all

Anti-Angiotensin II Type 1 Receptor antibody images

  • All lanes : Anti-Angiotensin II Type 1 Receptor antibody (ab18801) at 1/800 dilution

    Lane 1 : Dog heart whole tissue lysate at 50 µg
    Lane 2 : Dog heart whole tissue lysate at 25 µg
    Lane 3 : Dog heart whole tissue lysate at 10 µg
    Lane 4 : Dog heart whole tissue lysate at 5 µg
    Lane 5 : Dog heart whole tissue lysate at 2 µg

    Secondary
    HRP conjugated donkey polycloanl antibody

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 32 kDa (possible non-specific binding).

    Exposure time : 2 minutes

    This image is courtesy of an Abreview submitted by Dr sudhish mishra

    See Abreview

  • ab18801 staining Angiotensin II Type 1 in porcine kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 30 minutes at 20°C; antigen retrieval was by heat mediation with a citrate buffer. Samples were incubated with primary antibody (1/100 in PBS + Triton X-100 + goat serum) for 24 hours at 4°C. An HRP secondary antibody was used.

    See Abreview

  • ab18801 staining Angiotensin II Type 1 Receptor in Mouse kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 30 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in PBS + Triton X100) for 24 hours at 4°C. An undiluted HRP-conjugated anti-rabbit polyclonal was used as the secondary antibody.

    See Abreview

References for Anti-Angiotensin II Type 1 Receptor antibody (ab18801)

This product has been referenced in:
  • Wang J  et al. Qishenyiqi Dropping Pill attenuates myocardial fibrosis in rats by inhibiting RAAS-mediated arachidonic acid inflammation. J Ethnopharmacol 176:375-84 (2015). WB ; Rat . Read more (PubMed: 26590099) »
  • Chauhan VP  et al. Angiotensin inhibition enhances drug delivery and potentiates chemotherapy by decompressing tumour blood vessels. Nat Commun 4:2516 (2013). IHC-Fr ; Mouse . Read more (PubMed: 24084631) »

See all 12 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (Duodenum)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Universal decloaker
Permeabilization No
Specification Duodenum
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative Formaldehyde
Username

Anita Tsang

Verified customer

Submitted May 25 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native) (12.5% gel)
Sample Rat Tissue lysate - whole (bladder)
Specification bladder
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 23 2013

Gracias por contactarnos.

Este anticuerpo esta testado y garantizado para reconocer el receptor Angiotensin II Type 1 mediante WB, IHC-Fr, IHC-FoFr, IHC-P y microscopia electrónica en ratón, rata, bovino, canino y cerdo.
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Thank you for your enquiry.

I can confirm that ab18801 Angiotensin II Type 1 Receptor antibody detects both isoforms (1A and 1B). Therefore, the following two peptides will be suitable as blocking peptides:

Angiotensin II Type 1 R...

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Thank you for your email.

We did not receive any recent complaints for these lot so I am sorry we are unable to determine the cause of the problem. GR64320-2 is most recent batch for this product which was also failed along with GR13003-3 so I...

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Thank you for your email.

The protcol looks fine to me however could you please clarify the following;

- Is it true no antigen retrieval was used?
- Are the tissue sections frozen or paraffin embedded? If they were frozen then ace...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Mouse kidney)
Specification Mouse kidney
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citratbuffer
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Ms. Andrea Dannullis

Verified customer

Submitted Aug 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (kidney pig)
Specification kidney pig
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citratebuffer
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Ms. Andrea Dannullis

Verified customer

Submitted Aug 03 2012

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I am at...

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Thank you for your inquiry.

Immunohistochemistry results will vary with different tissue types and fixation protocols. We recommend testing a range of three dilutions, to determine which gives the most intense specific signal with the least ...

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