Anti-Angiotensin II Type 2 Receptor antibody [EPR3876] (ab92445)


  • Product name
    Anti-Angiotensin II Type 2 Receptor antibody [EPR3876]
    See all Angiotensin II Type 2 Receptor primary antibodies
  • Description
    Rabbit monoclonal [EPR3876] to Angiotensin II Type 2 Receptor
  • Host species
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Angiotensin II Type 2 Receptor aa 300-400 (C terminal). The exact sequence is proprietary.

  • Positive control
    • HepG2, 293T, HeLa and fetal liver lysates
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab92445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target


    • All lanes : Anti-Angiotensin II Type 2 Receptor antibody [EPR3876] (ab92445) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 41 kDa


    This product has been referenced in:
    • Ram RS  et al. Cancer Stem Cells in Moderately Differentiated Lip Squamous Cell Carcinoma Express Components of the Renin-Angiotensin System. Front Surg 4:30 (2017). WB ; Human . Read more (PubMed: 28634582) »
    • Khan N  et al. Attenuation of the Infiltration of Angiotensin II Expressing CD3+T-Cells and the Modulation of Nerve Growth Factor in Lumbar Dorsal Root Ganglia - A Possible Mechanism Underpinning Analgesia Produced by EMA300, An Angiotensin II Type 2 (AT2) Receptor Antagonist. Front Mol Neurosci 10:389 (2017). Read more (PubMed: 29200998) »

    See all 11 Publications for this product

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Western blot
    Pig Tissue lysate - whole (Newborn kidney)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    40 µg
    Newborn kidney
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Dec 28 2017

    AT2R is a 41 kDa protein that contains a large number of potential glycosylation sites, and depending on the amount of post-translational glycosylation, the protein may run at a variety of sizes between 41 and 58 kDa. The SwissProt link below depi...

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    Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    I would like to reassure you that this antibody is tested and covered by our 6 month guarantee forWB a...

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    According to our records, 92445 Anti-Angiotensin II Type 2 Receptor antibody [EPR3876] was proving difficult to use inWB and we were in contact in order to help resolve the issue.

    Looking at our correspondence, it appears that we are awaitin...

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    Yes of course, thanks for your reply.

    We don't have any specific recommendations for western blotting protocol for ab32212. Although a good starting dilution for western blotting using ab32212 is noted as 1:5000.

    ab52174 is antibody...

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    Thanks for your email.

    I'm sorry but I don't quite understand your question. What is it that you requested for ab32213 and ab52174?

    Thanks for the additional details.

    Thank you for your enquiry.

    i have checked and none ofthese 3 antibodies have a specific western blot protocol associated with them, although typically a 1:1000 dilution, or 1ug/ml primaryantibody concentration works well as a starting point ...

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    Thanks for your email.

    For ab11734 I'd recommend starting with a dilution of 1: 250 and dilute ab87436 to 1:1000. The primary antibody diluent could be TBS with 0.05% Tween 20.

    I hope this is helpful. Please contact me again if you ...

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    Thank you for your enquiry.

    Here is an antibody that has be used in western blotting and IHC on rat samples for ACE 1:

    Here is an antibody that has ...

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    Thanks for your enquiry. For western blotting, its recommended to use 1ug/ml as the starting concentration for ab2742. This may need to be optimized. For the antibody diluent, you can use TBS/0.5% Tween 20 (TBST). I hope this is helpful. Please let me ...

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