Anti-Angiotensinogen antibody [EPR2931] (ab108334)

Overview

  • Product nameAnti-Angiotensinogen antibody [EPR2931]
    See all Angiotensinogen primary antibodies
  • Description
    Rabbit monoclonal [EPR2931] to Angiotensinogen
  • Specificityab108334 is raised against the Human Angiotensinogen protein. This has little homology with mouse or rat Angiotensinogen and in our hands we do not get good results when testing in these species (in western blot the band is weak or absent and at a lower molecular weight than in human samples) therefore we cannot guarantee this product for use in these species. When using this antibody for western blot we recommend blocking with 5% BSA for best results. In our hands, blocking with milk does not give optimal results in WB and some lower molecular weight binding can be observed.
  • Tested applicationsSuitable for: WB, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (internal sequence)

  • Positive control
    • Human fetal artery, fetal heart, fetal liver, adult kidney, HepG2, 293T, and plasma lysates; HepG2 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

     

    Alternative versions available:

    Anti-Angiotensinogen antibody (Alexa Fluor® 647) [EPR2931] (ab198604)

    Anti-Angiotensinogen antibody (Alexa Fluor® 488) [EPR2931] (ab197901)

Applications

Our Abpromise guarantee covers the use of ab108334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 53 kDa.

Block with 5% BSA

IP 1/10 - 1/100.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IHC-P.
  • Target

    • FunctionEssential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. In response to lowered blood pressure, the enzyme renin cleaves angiotensinogen to produce angiotensin-1 (angiotensin 1-10). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2 (angiotensin 1-8). Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3 (angiotensin 2-8), angiotensin-4 (angiotensin 3-8). Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin). Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2.
      Angiotensin-2 acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone.
      Angiotensin-3 stimulates aldosterone release.
      Angiotensin 1-7 is a ligand for the G-protein coupled receptor MAS1 (By similarity). Has vasodilator and antidiuretic effects (By similarity). Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets.
    • Tissue specificityExpressed by the liver and secreted in plasma.
    • Involvement in diseaseGenetic variations in AGT are a cause of susceptibility to essential hypertension (EHT) [MIM:145500]. Essential hypertension is a condition in which blood pressure is consistently higher than normal with no identifiable cause.
      Defects in AGT are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
    • Sequence similaritiesBelongs to the serpin family.
    • Post-translational
      modifications
      Beta-decarboxylation of Asp-34 in angiotensin-2, by mononuclear leukocytes produces alanine. The resulting peptide form, angiotensin-A, has the same affinity for the AT1 receptor as angiotensin-2, but a higher affinity for the AT2 receptor.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Aangiotensinogen (serpin peptidase inhibitor clade A member 8) antibody
      • AGT antibody
      • AI265500 antibody
      • Alpha 1 antiproteinase antitrypsin antibody
      • Ang antibody
      • Ang I antibody
      • Ang II antibody
      • Ang III antibody
      • AngII antibody
      • Angiotensin I antibody
      • Angiotensin II antibody
      • Angiotensin III antibody
      • Angiotensin-3 antibody
      • Angiotensinogen (PAT) antibody
      • Angiotensinogen antibody
      • ANGT_HUMAN antibody
      • ANHU antibody
      • ANRT antibody
      • AT-2 antibody
      • AT-II antibody
      • Des-Asp[1]-angiotensin II antibody
      • FLJ92595 antibody
      • FLJ97926 antibody
      • MGC105326 antibody
      • PAT antibody
      • Pre angiotensinogen antibody
      • Serine (or cysteine) proteinase inhibitor antibody
      • Serpin A8 antibody
      • Serpin peptidase inhibitor clade A member 8 antibody
      • SERPINA8 antibody
      see all

    Anti-Angiotensinogen antibody [EPR2931] images

    • All lanes : Anti-Angiotensinogen antibody [EPR2931] (ab108334) at 1/1000 dilution

      Lane 1 : Mouse kidney whole cell lysate
      Lane 2 : Rat kidney whole cell lysate
      Lane 3 : Human kidney whole cell lysate
      Lane 4 : Mouse liver whole cell lysate
      Lane 5 : Rat liver whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit IgG (H+L) peroxidase conjugated. at 1/2000 dilution

      Predicted band size : 53 kDa


      Exposure time : 1 minute

      Ab108334 targeting angiotensinogen in various mouse, rat and human tissues (see lane descriptions).

      Blocking buffer for the first lanes 1-5: 5% NFDM/TBST; the second lanes 1-5: BSA/TBST.

    • All lanes : Anti-Angiotensinogen antibody [EPR2931] (ab108334) at 1/1000 dilution

      Lane 1 : Human fetal liver whole cell lysate
      Lane 2 : Human fetal liver whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit IgG (H+L) peroxidase conjugated. at 1/2000 dilution

      Predicted band size : 53 kDa


      Exposure time : 3 minutes

      Lane 1 blocking buffer: NFDM/TBST

      Lane 2 blocking buffer: BSA/TBST

    • All lanes : Anti-Angiotensinogen antibody [EPR2931] (ab108334) at 1/1000 dilution

      Lane 1 : Human fetal artery lysate
      Lane 2 : Human fetal heart lysate
      Lane 3 : Human fetal liver lysate
      Lane 4 : HepG2 lysate
      Lane 5 : 293T lysate
      Lane 6 : Human plasma lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 53 kDa
    • ab108334, at 1/100 dilution, staining Angiotensinogen in HepG2 cells by Immunofluorescence.

    References for Anti-Angiotensinogen antibody [EPR2931] (ab108334)

    This product has been referenced in:
    • Gorman JL  et al. Angiotensin II evokes angiogenic signals within skeletal muscle through co-ordinated effects on skeletal myocytes and endothelial cells. PLoS One 9:e85537 (2014). WB ; Rat, Human . Read more (PubMed: 24416421) »
    • Yan J  et al. Long-term effects of maternal diabetes on blood pressure and renal function in rat male offspring. PLoS One 9:e88269 (2014). WB ; A wide range of other invertebrates . Read more (PubMed: 24505458) »

    See all 5 Publications for this product

    Product Wall

    Application Western blot
    Sample Mouse Cell lysate - whole cell (Cardiomyocytes)
    Gel Running Conditions Reduced Denaturing (10% PAGE)
    Loading amount 25 µg
    Specification Cardiomyocytes
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 28 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cultured Cells (human embryonic stem cells)
    Specification human embryonic stem cells
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative Methanol
    Username

    Abcam user community

    Verified customer

    Submitted Jun 06 2013

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (epididymis)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
    Permeabilization No
    Specification epididymis
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative bouin's solution
    Username

    Abcam user community

    Verified customer

    Submitted Apr 09 2013

    Thank you for contacting us. Antibodies are OK at room temperature for about 1 a week. We use this time to ship antibodies to our customers although we include icepacks in order to keep them at 4C in case of any unnecessary delays. Once receive...

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    Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments.   There is a very clear, single band on the customer's blot at 34 kDa, though the predicted band of precursor 53 kDa.   During ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"