Recombinant
RabMAb

Anti-Angiotensinogen antibody [EPR2931] (ab108334)

Overview

  • Product name
    Anti-Angiotensinogen antibody [EPR2931]
    See all Angiotensinogen primary antibodies
  • Description
    Rabbit monoclonal [EPR2931] to Angiotensinogen
  • Specificity
    ab108334 is raised against the Human Angiotensinogen protein. This has little homology with mouse or rat Angiotensinogen and in our hands we do not get good results when testing in these species (in western blot the band is weak or absent and at a lower molecular weight than in human samples) therefore we cannot guarantee this product for use in these species. When using this antibody for western blot we recommend blocking with 5% BSA for best results. In our hands, blocking with milk does not give optimal results in WB and some lower molecular weight binding can be observed.
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (internal sequence)

  • Positive control
    • Human fetal artery, fetal heart, fetal liver, adult kidney, HepG2, 293T, and plasma lysates; HepG2 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab108334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 53 kDa.

Block with 5% BSA

IP 1/10 - 1/100.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function
      Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. In response to lowered blood pressure, the enzyme renin cleaves angiotensinogen to produce angiotensin-1 (angiotensin 1-10). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2 (angiotensin 1-8). Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3 (angiotensin 2-8), angiotensin-4 (angiotensin 3-8). Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin). Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2.
      Angiotensin-2 acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone.
      Angiotensin-3 stimulates aldosterone release.
      Angiotensin 1-7 is a ligand for the G-protein coupled receptor MAS1 (By similarity). Has vasodilator and antidiuretic effects (By similarity). Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets.
    • Tissue specificity
      Expressed by the liver and secreted in plasma.
    • Involvement in disease
      Genetic variations in AGT are a cause of susceptibility to essential hypertension (EHT) [MIM:145500]. Essential hypertension is a condition in which blood pressure is consistently higher than normal with no identifiable cause.
      Defects in AGT are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
    • Sequence similarities
      Belongs to the serpin family.
    • Post-translational
      modifications
      Beta-decarboxylation of Asp-34 in angiotensin-2, by mononuclear leukocytes produces alanine. The resulting peptide form, angiotensin-A, has the same affinity for the AT1 receptor as angiotensin-2, but a higher affinity for the AT2 receptor.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Aangiotensinogen (serpin peptidase inhibitor clade A member 8) antibody
      • AGT antibody
      • AI265500 antibody
      • Alpha 1 antiproteinase antitrypsin antibody
      • Ang antibody
      • Ang I antibody
      • Ang II antibody
      • Ang III antibody
      • AngII antibody
      • Angiotensin I antibody
      • Angiotensin II antibody
      • Angiotensin III antibody
      • Angiotensin-3 antibody
      • Angiotensinogen (PAT) antibody
      • Angiotensinogen antibody
      • ANGT_HUMAN antibody
      • ANHU antibody
      • ANRT antibody
      • AT-2 antibody
      • AT-II antibody
      • Des-Asp[1]-angiotensin II antibody
      • FLJ92595 antibody
      • FLJ97926 antibody
      • MGC105326 antibody
      • PAT antibody
      • Pre angiotensinogen antibody
      • Serine (or cysteine) proteinase inhibitor antibody
      • Serpin A8 antibody
      • Serpin peptidase inhibitor clade A member 8 antibody
      • SERPINA8 antibody
      see all

    Images

    • All lanes : Anti-Angiotensinogen antibody [EPR2931] (ab108334) at 1/1000 dilution

      Lane 1 : Mouse kidney whole cell lysate
      Lane 2 : Rat kidney whole cell lysate
      Lane 3 : Human kidney whole cell lysate
      Lane 4 : Mouse liver whole cell lysate
      Lane 5 : Rat liver whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit IgG (H+L) peroxidase conjugated. at 1/2000 dilution

      Predicted band size : 53 kDa


      Exposure time : 1 minute

      Ab108334 targeting angiotensinogen in various mouse, rat and human tissues (see lane descriptions).

      Blocking buffer for the first lanes 1-5: 5% NFDM/TBST; the second lanes 1-5: BSA/TBST.

    • All lanes : Anti-Angiotensinogen antibody [EPR2931] (ab108334) at 1/1000 dilution

      Lane 1 : Human fetal liver whole cell lysate
      Lane 2 : Human fetal liver whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit IgG (H+L) peroxidase conjugated. at 1/2000 dilution

      Predicted band size : 53 kDa


      Exposure time : 3 minutes

      Lane 1 blocking buffer: NFDM/TBST

      Lane 2 blocking buffer: BSA/TBST

    • All lanes : Anti-Angiotensinogen antibody [EPR2931] (ab108334) at 1/1000 dilution

      Lane 1 : Human fetal artery lysate
      Lane 2 : Human fetal heart lysate
      Lane 3 : Human fetal liver lysate
      Lane 4 : HepG2 lysate
      Lane 5 : 293T lysate
      Lane 6 : Human plasma lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 53 kDa
    • ab108334, at 1/100 dilution, staining Angiotensinogen in HepG2 cells by Immunofluorescence.

    References

    This product has been referenced in:
    • Xiang X  et al. Tuberous sclerosis complex 1-mechanistic target of rapamycin complex 1 signaling determines brown-to-white adipocyte phenotypic switch. Diabetes 64:519-28 (2015). Read more (PubMed: 25213336) »
    • Kemp JR  et al. Angiotensin II-regulated microRNA 483-3p directly targets multiple components of the renin-angiotensin system. J Mol Cell Cardiol 75:25-39 (2014). Read more (PubMed: 24976017) »

    See all 7 Publications for this product

    Customer reviews and Q&As

    Filter by Application

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    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Cardiomyocytes)
    Gel Running Conditions
    Reduced Denaturing (10% PAGE)
    Loading amount
    25 µg
    Specification
    Cardiomyocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 28 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cultured Cells (human embryonic stem cells)
    Specification
    human embryonic stem cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Methanol
    Username

    Abcam user community

    Verified customer

    Submitted Jun 06 2013

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (epididymis)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer
    Permeabilization
    No
    Specification
    epididymis
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    bouin's solution
    Username

    Abcam user community

    Verified customer

    Submitted Apr 09 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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