Overview

  • Product nameAnti-ANGPTL2 antibodySee all ANGPTL2 primary antibodies ...
  • Description
    Rabbit polyclonal to ANGPTL2
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human ANGPTL2.

    (Peptide available as ab36012.)

  • Positive control
    • This antibody gave a positive signal in the following lysate: Tagged ANGPTL2 recombinant protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab35574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1 µg/ml. Detects a band of approximately 79 (size of the recombinant protein + tag) kDa (predicted molecular weight: 57 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInduces sprouting in endothelial cells through an autocrine and paracrine action.
    • Tissue specificityWidely expressed in heart, small intestine, spleen and stomach. Also found in lower levels in colon, ovary, adrenal gland, skeletal muscle and in prostate.
    • Sequence similaritiesContains 1 fibrinogen C-terminal domain.
    • Post-translational
      modifications
      N-glycosylated.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Angiopoietin like 2 antibody
      • Angiopoietin related protein 2 antibody
      • Angiopoietin-like 2 antibody
      • Angiopoietin-like protein 2 antibody
      • Angiopoietin-related protein 2 antibody
      • ANGL2_HUMAN antibody
      • Angptl2 antibody
      • Arp2 antibody
      • HARP antibody
      • MGC8889 antibody
      • UNQ170/PRO196 antibody
      see all

    Anti-ANGPTL2 antibody images

    • Anti-ANGPTL2 antibody (ab35574) at 1 µg/ml + ANGPTL2 Recombinant Protein at 0.1 µg

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 57 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)

    References for Anti-ANGPTL2 antibody (ab35574)

    ab35574 has not yet been referenced specifically in any publications.

    Product Wall

    Abreviews
    Application Western blot
    Sample Mouse Cell lysate - whole cell (Mouse cell lysate)
    Loading amount 20 µg
    Specification Mouse cell lysate
    Gel Running Conditions Reduced Denaturing (8% SDS-Page gel with a 5% stacking gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Prof. Paul Townsend

    Verified customer

    Submitted Mar 24 2010

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (kidney)
    Specification kidney
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Mar 24 2010

    Application Western blot
    Sample Mouse Tissue lysate - whole (Intestine, kidney)
    Loading amount 20 µg
    Specification Intestine, kidney
    Gel Running Conditions Reduced Denaturing
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Mar 17 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"