Anti-ANGPTL2 antibody (ab36014)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab36014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1 µg/ml. Detects a band of approximately 80 (size of the recombinant protein + tag) kDa (predicted molecular weight: 57 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInduces sprouting in endothelial cells through an autocrine and paracrine action.
    • Tissue specificityWidely expressed in heart, small intestine, spleen and stomach. Also found in lower levels in colon, ovary, adrenal gland, skeletal muscle and in prostate.
    • Sequence similaritiesContains 1 fibrinogen C-terminal domain.
    • Post-translational
      modifications
      N-glycosylated.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Angiopoietin like 2 antibody
      • Angiopoietin related protein 2 antibody
      • Angiopoietin-like protein 2 antibody
      • Angiopoietin-related protein 2 antibody
      • ANGL2_HUMAN antibody
      • Angptl2 antibody
      • Arp2 antibody
      • HARP antibody
      • MGC8889 antibody
      • UNQ170/PRO196 antibody
      see all

    Anti-ANGPTL2 antibody images

    • Anti-ANGPTL2 antibody (ab36014) at 1 µg/ml + ANGPTL2 Recombinant Protein at 0.1 µg

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 57 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)

    References for Anti-ANGPTL2 antibody (ab36014)

    ab36014 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us.


    The antibody has not been tested against other proteins, to our knowledge. I aligned the immunogen sequence (*********) with mouse ANGPTL3 and ANGPTL4 and there was no significant homology.


    H...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Rat Cell lysate - whole cell (Rat Cardiomyoctes)
    Loading amount 20 µg
    Specification Rat Cardiomyoctes
    Gel Running Conditions Reduced Denaturing (8% SDS-Page gel with a 5% stacking gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Prof. Paul Townsend

    Verified customer

    Submitted Mar 24 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (Intestine, Kidney)
    Loading amount 20 µg
    Specification Intestine, Kidney
    Gel Running Conditions Reduced Denaturing
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Mar 12 2010

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (kidney)
    Specification kidney
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Mar 05 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"