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Rabbit polyclonal to ANGPTL3
Predicted to work with:
Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus ANGPTL3.
(Peptide available as
This antibody gave a positive signal in the following whole cell lysates:
HepG2; HEK293; A498
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 58 & 56 kDa (predicted molecular weight: 54 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Expressed principally in liver. Weakly expressed in kidney.
Contains 1 fibrinogen C-terminal domain.
Information by UniProt
ANG 5 antibody
Western blot - ANGPTL3 antibody (ab84035)
All lanes :
Anti-ANGPTL3 antibody (ab84035) at 1 µg/ml
Lane 1 :
HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 :
HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 :
A498 (Human Kidney Carcinoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size :
Observed band size :
58 kDa (
why is the actual band size different from the predicted?
Additional bands at :
56 kDa (possible cleavage fragment).
Exposure time :
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"