Overview

  • Product name
  • Description
    Rabbit polyclonal to ANGPTL3
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus ANGPTL3.

    (Peptide available as ab94376.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HepG2; HEK293; A498

Properties

Applications

Our Abpromise guarantee covers the use of ab84035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 58 & 56 kDa (predicted molecular weight: 54 kDa).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Tissue specificity
      Expressed principally in liver. Weakly expressed in kidney.
    • Sequence similarities
      Contains 1 fibrinogen C-terminal domain.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • ANG 5 antibody
      • ANG-5 antibody
      • ANG5 antibody
      • Angiopoietin 5 antibody
      • Angiopoietin like 3 antibody
      • Angiopoietin related protein 3 antibody
      • Angiopoietin-5 antibody
      • Angiopoietin-like protein 3 antibody
      • Angiopoietin-related protein 3 antibody
      • ANGL3_HUMAN antibody
      • ANGPT5 antibody
      • ANGPTL3 antibody
      • ANL3 antibody
      • FHBL2 antibody
      • OTTHUMP00000010719 antibody
      • UNQ153/PRO179 antibody
      see all

    Images

    • All lanes : Anti-ANGPTL3 antibody (ab84035) at 1 µg/ml

      Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 3 : A498 (Human Kidney Carcinoma) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 54 kDa
      Observed band size : 58 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 56 kDa (possible cleavage fragment).

      Exposure time : 20 minutes

    References

    ab84035 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab84035.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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