Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab5910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
IHC-P Use a concentration of 3 - 10 µg/ml.

Target

  • FunctionRequired for cytokinesis. Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression.
  • Tissue specificityUbiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors.
  • Sequence similaritiesContains 1 PH domain.
  • Developmental stageExpressed in fetal brain, heart, kidney, liver, lung, skeletal muscle, spleen and thymus. In dividing cells expression increases during S and G2 phases, peaks at mitosis and subsequently drops as cells enter G1 phase.
  • Post-translational
    modifications
    Phosphorylated during mitosis.
    Ubiquitinated, and this requires FZR1/CDH1.
  • Cellular localizationNucleus. Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Mainly found in the nucleus during interphase. Colocalizes with cortical F-actin upon nuclear envelope breakdown in mitosis and subsequently concentrates in the area of the prospective contractile ring in anaphase. This pattern persists until telophase, when the protein becomes concentrated in the midbody.
  • Information by UniProt
  • Database links
  • Alternative names
    • Actin binding protein scraps homolog Drosophila antibody
    • Actin binding protein anillin antibody
    • Actin-binding protein anillin antibody
    • Anillin actin binding protein antibody
    • ANILLIN antibody
    • ANLN antibody
    • ANLN_HUMAN antibody
    • DKFZp779A055 antibody
    • Scra antibody
    • Scraps antibody
    • Scraps homolog antibody
    see all

Anti-Anillin antibody images

  • ab14404 staining Anillinin Mouse fibroblast cellsby ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with methanol and blocked with 4% BSA for 2 hours at 4°C. Samples were incubated with primary antibody (1/25 in PBS + 1% BSA) for 2 hour at 25°C. An  Alexa Fluor®488-conjugated Donkey anti-goat  polyclonal(1/500) was used as the secondary antibody.

    See Abreview

  • ab5910 at 10ug/ml staining Anillin in human kidney tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in microwave and in Tris/EDTA buffer (pH 9.0). HRP-staining procedure was used for detection.

References for Anti-Anillin antibody (ab5910)

This product has been referenced in:
  • Stoeckelhuber M  et al. Human axillary apocrine glands: proteins involved in the apocrine secretory mechanism. Histol Histopathol 26:177-84 (2011). IHC-P ; Human . Read more (PubMed: 21154231) »
  • Hall PA  et al. The septin-binding protein anillin is overexpressed in diverse human tumors. Clin Cancer Res 11:6780-6 (2005). IHC-P ; Human . Read more (PubMed: 16203764) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry
Sample Mouse Cultured Cells (fibroblasts)
Specification fibroblasts
Fixative Paraformaldehyde
Permeabilization Yes - Methanol
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
Username

Dr. Manoj B Menon

Verified customer

Submitted Mar 22 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Immortalized Fibroblasts)
Specification Immortalized Fibroblasts
Fixative Paraformaldehyde
Permeabilization Yes - 0,25% Triton-X-100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Username

Dr. Manoj B Menon

Verified customer

Submitted Jan 22 2013

DISCOUNT CODE: xxxxxx
Expiration date: 07/05/13
Value: € 380
I am very pleased to hear you would like to accept our offer and test ab5910 in ICC on mouse samples. Because ICC is an untested application and mouse is also an untested species...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (hippocampal neurons)
Specification hippocampal neurons
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10
Username

Abcam user community

Verified customer

Submitted Jun 28 2006

Dear Professor Hall, Thank you for clarifying that I cannot use the published images without the correct approval! It would be really useful if you would be able to provide some images that we are able to show on our website. Other customers often ...

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Thank you for your enquiry. Ab5910 is a "Fast Track antibody:" Fast-Track antibodies are immunogen affinity purified and are shown by ELISA to have high titre values against their immunogen. We are currently testing them for use in Western blotting. Ab...

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Thank you for your enquiry and your patience. We are very pleased to hear that you have successfully used this product. However, we regret to inform you that we do not ship our products on dry ice especially not within Cambridge area. Did you ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"